Ramírez-Jiménez Rafael, Artiga Álvaro, Mitchell Scott G, Martín-Rapún Rafael, de la Fuente Jesús M
Instituto de Ciencia de Materiales de Aragón (CSIC-Universidad de Zaragoza), c/ Pedro Cerbuna s/n, 50009 Zaragoza, Spain.
Centro de Investigación Biomédica en Red in Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), 28029 Madrid, Spain.
Nanomaterials (Basel). 2020 Mar 17;10(3):539. doi: 10.3390/nano10030539.
Gold nanoprisms possess remarkable optical properties that make them useful for medical biotechnology applications such as diagnosis and photothermal therapy. However, shape-selective synthesis of gold nanoprisms is not trivial and typically requires either toxic surfactants or time-consuming purification protocols, which can limit their applicability. Here, we show how triangular gold nanoprisms of different sizes can be purified by precipitation using the non-toxic glutathione ligand, thereby removing the need for toxic surfactants and bottleneck purification techniques. The protocol is amenable for direct scaling up as no instrumentation is required in the critical purification step. The new purification method provides a two-fold increased yield in gold nanoprisms compared to electrophoretic filtration, while providing nanoprisms of similar localized surface plasmon resonance wavelength. Crucially, the gold nanoprisms isolated using this methodology show fewer non-specific interactions with cells and lower cellular internalization, which paves the way for a higher selectivity in therapeutic applications.
金纳米棱柱状颗粒具有卓越的光学特性，这使其在诸如诊断和光热疗法等医学生物技术应用中十分有用。然而，金纳米棱柱状颗粒的形状选择性合成并非易事，通常需要使用有毒表面活性剂或耗时的纯化方案，这可能会限制它们的适用性。在此，我们展示了如何使用无毒的谷胱甘肽配体通过沉淀法纯化不同尺寸的三角形金纳米棱柱状颗粒，从而无需使用有毒表面活性剂和繁琐的纯化技术。该方案易于直接扩大规模，因为在关键的纯化步骤中无需仪器设备。与电泳过滤相比，这种新的纯化方法使金纳米棱柱状颗粒的产量提高了两倍，同时提供具有相似局域表面等离子体共振波长的纳米棱柱状颗粒。至关重要的是，使用这种方法分离出的金纳米棱柱状颗粒与细胞的非特异性相互作用更少，细胞内化程度更低，这为治疗应用中更高的选择性铺平了道路。
