Instituto de Nanociencia de Aragon (INA), Universidad de Zaragoza, 50018 Zaragoza, Spain.
Langmuir. 2012 Jun 19;28(24):8965-70. doi: 10.1021/la204712u. Epub 2012 Feb 9.
The paper describes a novel and straightforward wet-chemical synthetic route to produce biocompatible single-crystalline gold tabular nanoparticles, herein called nanoprisms (NPRs) due to their characteristic shape. Besides the novelty of the method to produce NPRs with an unprecedented high yield, the synthesis avoids the use of highly toxic cetyltrimethylammonium bromide (CTAB), the most widely used surfactant for the synthesis of gold anisotropic nanoparticles such as nanorods or nanoprisms. The method presented here allows for tuning the edge length of NPRs in the range of 100-170 nm by adjusting the final concentration/molar ratio of gold salt and reducing agent (thiosulfate), while the thickness of NPRs remained constant (9 nm). Thus, the surface plasmon band of NPRs can be set along the near-infrared (NIR) range. The resulting NPRs were derivatized with heterobifunctional polyethylene glycol (PEG) and 4-aminophenyl β-D-glucopyranoside (glucose) chains to improve their stability and cellular uptake, respectively. The heating properties of colloidal solutions of NPRs upon 1064 nm light illumination were evaluated. As a proof of concept, the biocompatibility and suitability of functional NPRs as photothermal agents were studied in cell cultures. Due to their biocompatibility (avoiding CTAB), ease of production, ease of functionalization, and remarkable heating features, the NPRs discussed herein represent a significant advance in the biocompatibility of nanoparticles and serve as an attractive alternative to those currently in use as plasmonic photothermal agents.
本文描述了一种新颖且直接的湿化学合成路线，用于生产生物相容性的单晶金板状纳米粒子，由于其独特的形状，我们将其称为纳米棱柱（NPR）。除了该方法生产具有空前高产率的 NPR 的新颖性之外，该合成还避免了使用高毒性的十六烷基三甲基溴化铵（CTAB），CTAB 是用于合成金各向异性纳米粒子（如纳米棒或纳米棱柱）的最广泛使用的表面活性剂。这里提出的方法允许通过调整金盐和还原剂（硫代硫酸盐）的最终浓度/摩尔比来调节 NPR 的边长在 100-170nm 范围内，而 NPR 的厚度保持不变（9nm）。因此，可以将 NPR 的表面等离子体带设置在近红外（NIR）范围内。所得的 NPR 用异双官能聚乙二醇（PEG）和 4-氨基苯-β-D-吡喃葡萄糖苷（葡萄糖）链进行衍生化，分别提高其稳定性和细胞摄取能力。评估了 NPR 胶体溶液在 1064nm 光照射下的加热特性。作为概念验证，研究了功能化 NPR 作为光热剂在细胞培养中的生物相容性和适用性。由于其生物相容性（避免 CTAB）、易于生产、易于功能化以及显著的加热特性，本文讨论的 NPR 代表了纳米粒子生物相容性的重大进展，并且是目前作为等离子体光热剂使用的那些的有吸引力的替代品。
