Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
Physiol Res. 2020 Apr 30;69(2):347-252. doi: 10.33549/physiolres.934313. Epub 2020 Mar 23.
Increased plasma cholesterol levels are listed between the major atherosclerosis risk factors. The final plasma cholesterol levels result from the interplay between the genetic and environmental (diet, physical activity) factors. Little is known, how dietary factor influence epigenetics. We have analyzed, if an over-generation feeding of rat with cholesterol influences total liver-DNA methylation, and if total liver-DNA methylation differ between the different rat strains (Prague hereditary hypercholesterolemic rats, Prague hereditary hypertriglyceridemic rats and Wistar Kyoto rats). The animals were feed with high fat (additional 5 % over normal capacity) high cholesterol (2 %) diet for 14 days. DNA methylation in the liver tissue in different generations was analyzed using the liquid chromatography coupled with tandem mass spectrometry. We have not observed any significant changes in total liver-DNA methylation over the 9 generations of animals feed by fat/cholesterol enriched diet. Additionally, there were no differences in DNA methylation between different rat strains. In animal model, the dietary changes (hypercholesterolemic diet) not significantly influence the total DNA methylation status within the liver.
血浆胆固醇水平升高被列为主要的动脉粥样硬化危险因素之一。最终的血浆胆固醇水平是由遗传和环境（饮食、体力活动）因素相互作用的结果。人们对饮食因素如何影响表观遗传学知之甚少。我们分析了，如果用胆固醇对大鼠进行过度几代的喂养，是否会影响肝脏总 DNA 甲基化，以及不同大鼠品系（布拉格遗传性高胆固醇血症大鼠、布拉格遗传性高甘油三酯血症大鼠和 Wistar Kyoto 大鼠）之间的总肝 DNA 甲基化是否存在差异。这些动物用高脂肪（比正常能力多 5%）高胆固醇（2%）饮食喂养 14 天。使用液相色谱-串联质谱法分析不同代动物肝脏组织中的 DNA 甲基化。我们没有观察到脂肪/胆固醇富集饮食喂养的 9 代动物的总肝 DNA 甲基化有任何显著变化。此外，不同大鼠品系之间的 DNA 甲基化没有差异。在动物模型中，饮食变化（高胆固醇血症饮食）并没有显著影响肝脏内的总 DNA 甲基化状态。
