Shima K, Ito N, Abe F, Hirota M, Yano M, Yamamoto Y, Uchida T, Noguchi K
Department of Laboratory Medicine, Tokushima University School of Medicine, Japan.
Diabetologia. 1988 Aug;31(8):627-31. doi: 10.1007/BF00264772.
A method for determination of serum glycated albumin by high-performance liquid chromatography is presented. The system involves anion exchange chromatography to separate albumin and consecutive boronate affinity chromatography to separate glycated and nonglycated albumin. The method is rapid (20 min), precise (coefficient of variation, 0.7-4.9%), requires only a small sample (5 microliters), and can be automated. Assay of glycated albumin by this method is not influenced by the protein concentration of the sample or the presence of glucose. The variation in glycated albumin values in consecutive samples obtained within a day from diabetic patients (coefficient of variation, 2.02 +/- 0.65%) was significantly smaller (p less than 0.001) than that of values for fructosamine (coefficient of variation, 4.33 +/- 2.0%). The values of glycated albumin in normal subjects (20.2 +/- 1.6%) were clearly less than those in diabetic patients [39.6 +/- 5.4% in 40 Type 1 (insulin-dependent) and 39.4 +/- 5.9% in 25 Type 2 (non-insulin-dependent) patients]. The serum glycated albumin level was well correlated with HbA1c in 65 diabetic patients (r = 0.60). Because the life span of albumin in the circulation is short, measurement of glycated albumin should be useful as a short-term index of glycaemic control.
本文介绍了一种用高效液相色谱法测定血清糖化白蛋白的方法。该系统包括用于分离白蛋白的阴离子交换色谱法以及用于分离糖化和非糖化白蛋白的连续硼酸亲和色谱法。该方法快速（20分钟）、精确（变异系数为0.7 - 4.9%），仅需少量样品（5微升），且可实现自动化。用该方法测定糖化白蛋白不受样品蛋白质浓度或葡萄糖存在与否的影响。糖尿病患者一天内连续采集的样品中糖化白蛋白值的变异（变异系数为2.02±0.65%）显著小于果糖胺值的变异（变异系数为4.33±2.0%）（p<0.001）。正常受试者的糖化白蛋白值（20.2±1.6%）明显低于糖尿病患者[40例1型（胰岛素依赖型）患者为39.6±5.4%，25例2型（非胰岛素依赖型）患者为39.4±5.9%]。65例糖尿病患者的血清糖化白蛋白水平与糖化血红蛋白A1c高度相关（r = 0.60）。由于循环中白蛋白的寿命较短，测定糖化白蛋白应可作为血糖控制的短期指标。
