Alesa Gyles Desireé, Pereira Júnior Anivaldo Duarte, Diniz Castro Lorena, Santa Brigida Andressa, Nobre Lamarão Maria Louze, Ramos Barbosa Wagner Luiz, Carréra Silva Júnior José Otávio, Ribeiro-Costa Roseane Maria
Laboratory of Pharmaceutical Nanotechnology, College of Pharmacy, Federal University of Pará, Belém 66075-110, Brazil.
Laboratory of Pharmaceutical and Cosmetic R&D, College of Pharmacy, Federal University of Pará, Belém 66075-110, Brazil.
Polymers (Basel). 2020 Mar 19;12(3):690. doi: 10.3390/polym12030690.
Chronic wounds are severe breaks in the skin barrier that fail to heal in an acceptable time-frame, thus preventing the complete restoration of the tissue's anatomical and functional integrity, increasing the likelihood of infections and apoptosis. Hydrogels are known as a drug delivery system and have the potential to cover wounds and burns on the skin. contains over 75 different bioactive compounds which are responsible for its anti-inflammatory and antimicrobial properties. In this study, the polyacrylamide-co-methylcellulose hydrogel containing were developed. The extract was prepared from lyophilized , using methanolic extraction, characterized by high performance liquid chromatography and incorporated into the hydrogels. These hydrogels were characterized by degree of swelling, Fourier-transform infrared spectroscopy, scanning electron microscopy, and thermal profiling using thermogravimetric analysis. The minimum inhibitory concentration test was done on the extract to evaluate its antibacterial and antifungal activity in vitro. The hydrogels and were shown to swell to almost 2000% of their original sizes. The Fourier-transform infrared spectroscopy indicated the presence of bands characteristic of and hydrogel polymers. The basic hydrogel showed greater thermal stability than the hydrogels with . The minimum inhibitory concentration showed inhibition of the growth of and spp. at specific concentrations. The hydrogel therefore presents itself as an excellent potential curative cover of cutaneous lesions.
慢性伤口是皮肤屏障的严重破损,在可接受的时间范围内无法愈合,从而阻碍了组织解剖结构和功能完整性的完全恢复,增加了感染和细胞凋亡的可能性。水凝胶作为一种药物递送系统而闻名,有覆盖皮肤伤口和烧伤的潜力。[提取物名称]含有75种以上不同的生物活性化合物,这些化合物赋予了它抗炎和抗菌特性。在本研究中,制备了含有[提取物名称]的聚丙烯酰胺 - 共 - 甲基纤维素水凝胶。该提取物由冻干的[提取物来源]经甲醇提取制得,通过高效液相色谱进行表征,并掺入水凝胶中。这些水凝胶通过溶胀度、傅里叶变换红外光谱、扫描电子显微镜以及使用热重分析的热分析进行表征。对[提取物名称]提取物进行了最低抑菌浓度测试,以评估其体外抗菌和抗真菌活性。含有[提取物名称]的水凝胶显示溶胀至其原始尺寸的近2000%。傅里叶变换红外光谱表明存在[提取物名称]和水凝胶聚合物的特征谱带。基础水凝胶显示出比含有[提取物名称]的水凝胶更高的热稳定性。最低抑菌浓度表明在特定浓度下对[细菌名称]和[真菌名称]菌株的生长有抑制作用。因此,该水凝胶是一种极具潜力的皮肤损伤治疗性敷料。