Laboratory of Research in Applied Microbiology, University of Franca, Franca, SP, Brazil.
Nucleus of Research in Sciences and Technology, University of Franca, Franca, SP, Brazil.
Anaerobe. 2020 Jun;63:102194. doi: 10.1016/j.anaerobe.2020.102194. Epub 2020 Mar 20.
Being aware of the remarkable antimicrobial potential of S. officinalis L., we aimed to evaluate the antimicrobial activity of the S. officinalis dichloromethane crude extract (SOD), dichloromethane-soluble fractions (SODH and SODD), SODD subfractions (SODD1 and SODD2), and pure substances (manool, salvigenin, and viridiflorol) against periodontopathogens. This bioassay-guided study comprises five antimicrobial tests-determination of the Minimum Inhibitory Concentration (MIC), determination of the Minimum Bactericidal Concentration (MBC), determination of the antibiofilm activity, construction of the Time-kill curve (determination of Bactericidal Kinetics), and determination of the Fractional Inhibitory Concentration Index-on six clinical bacterial isolates and three standard bacterial strains involved in periodontal disease. SOD has moderate activity against most of the tested bacteria, whereas SODD1, SODH1, SODH3, and manool afford the lowest results. The Porphyromonas gingivalis (ATTC and clinical isolate) biofilm is considerably resistant to all the samples. In association with chlorhexidine gluconate, only SODH1 exerts additive action against P. gingivalis (clinical isolate). Therefore, SODH1 and manool are promising antibacterial agents and may provide therapeutic solutions for periodontal infections.
鉴于贯叶连翘具有显著的抗菌潜力，我们旨在评估贯叶连翘二氯甲烷粗提物（SOD）、二氯甲烷可溶部分（SODH 和 SODD）、SODD 亚组分（SODD1 和 SODD2）以及纯物质（马鞭草醇、圣草酚和芫花素）对牙周病原菌的抗菌活性。这项基于生物测定的研究包括五个抗菌测试——最小抑菌浓度（MIC）的测定、最小杀菌浓度（MBC）的测定、抗生物膜活性的测定、时间杀伤曲线的构建（杀菌动力学的测定）以及分数抑菌浓度指数的测定，共针对六种临床分离细菌和三种与牙周病相关的标准细菌菌株进行。SOD 对大多数测试细菌具有中等活性，而 SODD1、SODH1、SODH3 和马鞭草醇的效果最低。牙龈卟啉单胞菌（ATTC 和临床分离株）生物膜对所有样品都具有相当的抗性。与洗必泰葡萄糖酸盐联合使用时，只有 SODH1 对牙龈卟啉单胞菌（临床分离株）具有相加作用。因此，SODH1 和马鞭草醇是有前途的抗菌剂，可能为牙周感染提供治疗解决方案。
