Department of Disease Control, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK.
Centre de Formation et de Recherche en Sante Rurale de Maferinyah, Conakry, Republic of Guinea.
Parasit Vectors. 2020 Mar 24;13(1):150. doi: 10.1186/s13071-020-04023-3.
Several mosquito collection methods are routinely used in vector control programmes. However, they target different behaviours causing bias in estimation of species diversity and abundance. Given the paucity of mosquito trap data in West Africa, we compared the performance of five trap-lure combinations and Human Landing Catches (HLCs) in Guinea.
CDC light traps (LT), BG sentinel 2 traps (BG2T), gravid traps (GT) and Stealth traps (ST) were compared in a 5 × 5 Latin Square design in three villages in Guinea between June and July 2018. The ST, a portable trap which performs similarly to a LT but incorporates LEDs and incandescent light, was included since it has not been widely tested. BG2T were used with BG and MB5 lures instead of CO to test the efficacy of these attractants. HLCs were performed for 5 nights, but not as part of the Latin Square. A Generalised Linear Mixed Model was applied to compare the effect of the traps, sites and collection times on mosquito abundance. Species identification was confirmed using PCR-based analysis and Sanger sequencing.
A total of 10,610 mosquitoes were captured across five traps. ST collected significantly more mosquitoes (7096) than the rest of the traps, but resulted in a higher number of damaged specimens. ST and BG2T collected the highest numbers of Anopheles gambiae (s.l.) and Aedes aegypti mosquitoes, respectively. HLCs captured predominantly An. coluzzii (41%) and hybrids of An. gambiae and An. coluzzii (36%) in contrast to the five traps, which captured predominantly An. melas (83%). The rural site (Senguelen) presented the highest abundance of mosquitoes and overall diversity in comparison with Fandie (semi-rural) and Maferinyah Centre I (semi-urban). Our results confirm the presence of four species for the first time in Guinea.
ST collected the highest number of mosquitoes suggesting this trap may play an important role for mosquito surveillance in Guinea and similar sites in West Africa. We recommend the incorporation of molecular tools in entomological studies since they have helped to identify 25 mosquito species in this area.
几种蚊子收集方法在病媒控制项目中经常使用。然而，它们针对的是不同的行为，这导致了物种多样性和丰度估计的偏差。鉴于西非蚊子诱捕器数据的缺乏，我们比较了五种诱捕器-诱饵组合和人诱捕（HLC）在几内亚的表现。
在 2018 年 6 月至 7 月期间，在几内亚的三个村庄中，使用 CDC 灯诱捕器（LT）、BG 哨兵 2 诱捕器（BG2T）、诱卵器（GT）和 Stealth 诱捕器（ST）进行了 5×5 拉丁方设计比较。ST 是一种便携式诱捕器，其性能与 LT 相似，但采用了 LED 和白炽灯光，由于尚未广泛测试，因此包括在内。BG2T 与 BG 和 MB5 诱饵一起使用，而不是 CO，以测试这些引诱剂的效果。HLC 进行了 5 个晚上，但不作为拉丁方的一部分。应用广义线性混合模型比较了诱捕器、地点和收集时间对蚊子丰度的影响。使用基于 PCR 的分析和 Sanger 测序确认了物种鉴定。
共捕获了 10610 只蚊子，涉及五种诱捕器。ST 捕获的蚊子数量明显多于其他诱捕器（7096 只），但造成的损坏标本数量也更多。ST 和 BG2T 分别捕获了最多的冈比亚按蚊（s.l.）和埃及伊蚊。HLC 捕获的主要是 An. coluzzii（41%）和 An. gambiae 和 An. coluzzii 的杂交种（36%），而不是五个诱捕器捕获的主要是 An. melas（83%）。与 Fandie（半农村）和 Maferinyah Centre I（半城市）相比，农村地点（Senguelen）的蚊子数量和整体多样性最高。我们的结果首次证实了几内亚存在四种蚊子。
ST 捕获的蚊子数量最多，这表明这种诱捕器可能在几内亚和西非类似地区的蚊子监测中发挥重要作用。我们建议在昆虫学研究中纳入分子工具，因为它们帮助确定了该地区的 25 种蚊子。
