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在伊朗使用聚合酶链反应检测泛冠状病毒(首次报告)。

Detection of pancoronavirus using PCR in in Iran (first report).

作者信息

Khalili Bagaloy Heydar, Sakhaee Ehsanollah, Khalili Mohammad

机构信息

1Department of Clinical Sciences, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.

2Department of Pathobiology, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.

出版信息

Comp Clin Path. 2017;26(1):193-196. doi: 10.1007/s00580-016-2368-0. Epub 2016 Nov 18.

Abstract

is a colossal family of viruses that cause a variety of diseases in humans and other animals. As of late, a novel coronavirus, not anterior-optically discerned in humans, has been identified in a denizen of the Middle East. There is growing evidence that the is host species for the virus and plays an important role of a source of human infection. Along these lines, the authors decided to detect coronaviruses in dromedary camels in two high-risk areas of Iran by employing an reverse transcription polymerase chain reaction (RT-PCR) assay. In the present study, nasal swab specimens were collected from 98 camels () traditionally reared in southeast and northwest of Iran. The detection of pancoronavirus was carried out, using RT-PCR. Pancoronavirus RNA was observed in seven cases among 98 nasal swab samples. Among these, 4 positive samples belonged to Azerbaijan province located in northwest of Iran and 3 positive samples were taken from southeast of Iran. The results of this study contribute to raising the hypothesis to the extent of transmission and risk factors for human infection and public health in Iran.

摘要

冠状病毒是一个庞大的病毒家族,可在人类和其他动物中引发多种疾病。最近,在中东地区发现了一种新型冠状病毒,此前在人类中未被光学检测到。越来越多的证据表明,单峰骆驼是该病毒的宿主物种,并在人类感染源方面发挥着重要作用。基于此,作者决定通过逆转录聚合酶链反应(RT-PCR)检测伊朗两个高风险地区单峰骆驼中的冠状病毒。在本研究中,从伊朗东南部和西北部传统饲养的98头骆驼()中采集了鼻拭子样本。使用RT-PCR进行全冠状病毒检测。在98份鼻拭子样本中,有7例检测到全冠状病毒RNA。其中,4份阳性样本来自伊朗西北部的阿塞拜疆省,3份阳性样本来自伊朗东南部。本研究结果有助于将关于伊朗人类感染的传播范围和危险因素以及公共卫生的假设提升到更高水平。

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