Department of Periodontics and community dentistry, college of dentistry, King Khalid University, Abha 62529, Saudi Arabia.
Centre for Stem Cell Research and Dept. of Microbiology and Clinical Parasitology, College of Medicine, King Khalid University, Abha 62529, Saudi Arabia.
Medicina (Kaunas). 2020 Mar 25;56(4):148. doi: 10.3390/medicina56040148.
: The study aimed to investigate the effect of bone marrow mesenchymal stromal cells (BMMSCs) on implant-bone osseointegration in type I diabetic New Zealand rabbits. BMMSCs harvested from healthy rabbits were processed and validated for purity and osteocyte differentiability. Mandibular incisors of diabetic and control rabbits were carefully extracted, and the sockets were plugged with collagen sponges. Platelet-rich plasma (PRP) containing osteoinductive BMMSCs, and plain PRP were injected into the collagen sponge of the right and left sockets respectively. Dental implants of 2.6 mm diameter and 10 mm length were inserted into the collagen sponge of both sockets. All the animals were sacrificed six weeks post surgery to evaluate an early stage of osseointegration; the mandibles scanned by X-ray microcomputed tomography (μCT) and subjected to 3D analysis. The μCT parameters of the right implant were paired against that of the left side of each animal and analyzed by paired T-test. The preclinical evaluation of the viability and osteocyte differentiation of the BMMSCs were consistent between both the donor samples. The osseointegration of dental implants with stem cell therapy (BMMSCs + PRP + collagen) in normal and diabetic rabbits was significantly higher than that of implants with adjunctive PRP + collagen only ( < 0.05). Stem Cell therapy with osteoinductive BMMSCs and PRP can offer a novel approach to enhance the osseointegration of dental implants in uncontrolled diabetic patients.
: 本研究旨在探讨骨髓间充质干细胞（BMMSCs）对 I 型糖尿病新西兰兔种植体骨整合的影响。从健康兔中提取 BMMSCs，进行纯度和成骨细胞分化能力的处理和验证。糖尿病和对照组兔的下颌切牙被小心地拔出，牙槽窝用胶原海绵填塞。富含血小板的血浆（PRP）中含有诱导成骨的 BMMSCs 和普通 PRP 分别注入右侧和左侧牙槽窝的胶原海绵中。直径 2.6 毫米、长 10 毫米的牙种植体插入双侧牙槽窝的胶原海绵中。术后 6 周处死所有动物，以评估骨整合的早期阶段；对下颌骨进行 X 射线微计算机断层扫描（μCT）扫描并进行 3D 分析。通过配对 T 检验对右侧种植体的 μCT 参数与每个动物左侧的参数进行配对分析。供体样本之间的 BMMSCs 活力和成骨细胞分化的临床前评估一致。与单独使用辅助 PRP+胶原的种植体相比，干细胞治疗（BMMSCs+PRP+胶原）的种植体的骨整合明显更高（<0.05）。具有成骨诱导作用的 BMMSCs 和 PRP 的干细胞治疗可为增强不受控制的糖尿病患者的牙种植体骨整合提供一种新方法。
