Xing Helin, Wang Xing, Xiao Saisong, Zhang Guilan, Li Meng, Wang Peihuan, Shi Quan, Qiao Pengyan, E Lingling, Liu Hongchen
Institute of Stomatology, Chinese PLA General Hospital, Beijing.
Hospital of Stomatology, Shanxi Medical University, Taiyuan.
Int J Nanomedicine. 2017 Oct 19;12:7709-7720. doi: 10.2147/IJN.S148001. eCollection 2017.
Titanium implant is a widely used method for dental prosthesis restoration. Nevertheless, in patients with systemic diseases, including osteoporosis, diabetes, and cancer, the success rate of the implant is greatly reduced. This study investigates a new implant material loaded with insulin-like growth factor 1 (IGF1), which could potentially improve the implant success rate, accelerate the occurrence of osseointegration, and provide a new strategy for implant treatment in osteoporotic patients.
Biofunctionalized polyelectrolyte multilayers (PEMs) with polyethylenimine as the excitation layer and gelatin/chitosan loaded with IGF1 were prepared on the surface of titanium implant by layer-by-layer self-assembly technique. The physical and chemical properties of the biofunctionalized PEMs, the biological characteristics of bone marrow mesenchymal stem cells (BMMSCs), and bone implant contact correlation test indexes were detected and analyzed in vitro and in vivo using osteoporosis rat model.
PEMs coatings loaded with IGF1 (TNS-PEM-IGF1-100) implant promoted the early stage of BMMSCs adhesion. Under the action of body fluids, the active coating showed sustained release of growth factors, which in turn promoted the proliferation and differentiation of BMMSCs and the extracellular matrix. At 8 weeks from implant surgery, the new bone around the implants was examined using micro-CT and acid fuchsin/methylene blue staining. The new bone formation increased with time in each group, while the TNS-PEM-IGF1-100 group showed the highest thickness and continuity.
TNS-PEM-IGF1-100 new implants can promote osseointegration in osteoporotic conditions both in vivo and in vitro and provide a new strategy for implant repair in osteoporotic patients.
钛植入物是一种广泛应用于牙修复的方法。然而，在患有包括骨质疏松症、糖尿病和癌症在内的全身性疾病的患者中，植入物的成功率会大大降低。本研究调查了一种负载胰岛素样生长因子1（IGF1）的新型植入材料，其可能提高植入成功率，加速骨整合的发生，并为骨质疏松症患者的植入治疗提供新策略。
采用层层自组装技术，在钛植入物表面制备以聚乙烯亚胺为激发层、负载IGF1的明胶/壳聚糖的生物功能化聚电解质多层膜（PEMs）。使用骨质疏松大鼠模型在体外和体内检测并分析生物功能化PEMs的物理和化学性质、骨髓间充质干细胞（BMMSCs）的生物学特性以及骨植入物接触相关测试指标。
负载IGF1（TNS-PEM-IGF1-100）的植入物的PEMs涂层促进了BMMSCs的早期黏附。在体液作用下，活性涂层显示出生长因子的持续释放，进而促进了BMMSCs的增殖和分化以及细胞外基质的形成。在植入手术8周后，使用显微CT和酸性品红/亚甲蓝染色检查植入物周围的新骨。每组新骨形成均随时间增加，而TNS-PEM-IGF1-100组显示出最高的厚度和连续性。
TNS-PEM-IGF1-100新型植入物在体内和体外均可促进骨质疏松条件下的骨整合，并为骨质疏松症患者的植入修复提供新策略。
