Kim Jeesoo, Nguyen Thi Thanh Hanh, Jin Juhui, Septiana Iis, Son Gyu-Min, Lee Gang-Hee, Jung You-Jin, Qureshi Dilshad, Mok Il Kyoon, Pal Kunal, Yang Soo-Yeon, Kim Seong-Bo, Kim Doman
1Graduate School of International Agricultural Technology and Institutes of Green Bioscience & Technology, Seoul National University, Seoul, Pyeongchang, 25354 Korea.
2Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, India.
Biotechnol Bioprocess Eng. 2019;24(2):282-287. doi: 10.1007/s12257-018-0408-0. Epub 2019 May 18.
plays an important role in the development of dental caries in humans by synthesizing adhesive insoluble glucans from sucrose by mutansucrase activity. To explore the anti-cariogenic characteristics of rubusoside (Ru), a natural sweetener component in (Rosaceae), we investigated the inhibitory effect of Ru against the activity of mutansucrase and the growth of Ru (50 mM) showed 97% inhibitory activity against 0.1 U/mL mutansucrase of with 500 mM sucrose. It showed competitive inhibition with a value of 1.1 ± 0.2 mM and IC of 2.3 mM. Its inhibition activity was due to hydrophobic and hydrogen bonding interactions based on molecular docking analysis. Ru inhibited the growth of as a bacteriostatic agent, with MIC and MBC values of 6 mM and 8 mM, respectively. In addition, Ru showed synergistic anti-bacterial activity when it was combined with curcumin. Therefore, Ru is a natural anti-cariogenic agent with anti-mutansucrase activity and antimicrobial activity against .
The online version of this article (doi: 10.1007/s12257-018-0408-0) contains supplementary material, which is available to authorized users.
通过变聚糖酶活性从蔗糖合成粘性不溶性葡聚糖，在人类龋齿发展中起重要作用。为了探索悬钩子苷（Ru）（蔷薇科中的一种天然甜味剂成分）的抗龋特性，我们研究了Ru对变聚糖酶活性和变形链球菌生长的抑制作用。Ru（50 mM）对含500 mM蔗糖的变形链球菌0.1 U/mL变聚糖酶显示出97%的抑制活性。它表现出竞争性抑制，Ki值为1.1±0.2 mM，IC50为2.3 mM。基于分子对接分析，其抑制活性归因于疏水和氢键相互作用。Ru作为抑菌剂抑制变形链球菌的生长，MIC和MBC值分别为6 mM和8 mM。此外，Ru与姜黄素联合时显示出协同抗菌活性。因此，Ru是一种具有抗变聚糖酶活性和抗变形链球菌活性的天然抗龋剂。
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