State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, 510000, China.
Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
Proteomics. 2020 Jul;20(13):e1900271. doi: 10.1002/pmic.201900271. Epub 2020 Apr 30.
In recent years, the emergence of single-cell omics technologies, which can profile genomics, transcriptomics, epigenomics, and proteomics, has provided unprecedented insights into characteristics of cancer, enabling higher resolution and accuracy to decipher the cellular and molecular mechanisms relating to tumorigenesis, evolution, metastasis, and immune responses. Single-cell multi-omics technologies, which are developed based on the combination of multiple single-cell mono-omics technologies, can simultaneously analyze RNA expression, single nucleotide polymorphism, epigenetic modification, or protein abundance, enabling the in-depth understanding of gene expression regulatory mechanisms. In this review, the state-of-the-art single-cell multi-omics technologies are summarized and the prospects of their application in cancer biology are discussed.
近年来，单细胞组学技术的出现，能够描绘基因组学、转录组学、表观基因组学和蛋白质组学，为癌症的特征提供了前所未有的见解，使更高的分辨率和准确性能够破译与肿瘤发生、进化、转移和免疫反应相关的细胞和分子机制。单细胞多组学技术是基于多种单细胞单组学技术的结合而发展起来的，它可以同时分析 RNA 表达、单核苷酸多态性、表观遗传修饰或蛋白质丰度，从而深入了解基因表达调控机制。本文总结了最新的单细胞多组学技术，并讨论了它们在癌症生物学中的应用前景。
