Clinic of Small Animal Medicine, LMU University of Munich, Munich, Germany.
Institute for Infectious Diseases and Zoonoses, LMU University of Munich, Munich, Germany.
J Feline Med Surg. 2021 Feb;23(2):181-184. doi: 10.1177/1098612X20913352. Epub 2020 Mar 31.
Inhalation chambers are commonly used for the delivery of aerosol drugs to cats with respiratory disease. The aim of the study was to identify successful cleaning methods for inhalation devices after standardised bacterial contamination.
Spacer devices of two different manufacturers were used: RC Chamber (Cegla Medizintechnik) and Aerokat (Trudell Medical International). The bacterial contamination was performed using . Previously marked areas of the chamber were contaminated with 50 μl of bacterial solution, containing between 2.2 ×10 and 2.1 ×10 colony-forming units/ml each. After cleaning the devices as recommended by each manufacturer (RC Chamber: special microwave cleaning bag [n = 5] or boiling water with liquid dish detergent for 15 mins [n = 5]; Aerokat: rinsing in a solution of lukewarm water and liquid dish detergent for 15 mins), chambers were air-dried for 24 h and samples for bacterial culture were taken from three defined areas. Sample material was applied on Müller-Hinton agar plates and subsequently incubated for 24 h at 37°C.
Bacterial contamination was not detected in any of the examined inhalation devices using the recommended cleaning methods.
If inhalation chambers are cleaned following the manufacturers' recommendations, successful bacterial decontamination can be expected.
吸入室常用于向患有呼吸道疾病的猫输送气溶胶药物。本研究的目的是确定在标准化细菌污染后，吸入装置的成功清洁方法。
使用了两种不同制造商的喷雾器设备：RC 室（Cegla Medizintechnik）和 Aerokat（特鲁德尔医疗国际公司）。使用. 对腔室先前标记的区域进行细菌污染，每个区域用 50μl 细菌溶液污染，其中每个腔室含有 2.2×10 和 2.1×10 个菌落形成单位/ml。按照每个制造商的建议清洁设备（RC 室：特殊微波清洁袋[ n = 5]或用液体餐具洗涤剂在沸水中煮沸 15 分钟[n = 5]；Aerokat：在微温的水和液体餐具洗涤剂的溶液中冲洗 15 分钟）后，腔室在空气中干燥 24 小时，并从三个确定的区域采集用于细菌培养的样本。将样本材料涂抹在 Mueller-Hinton 琼脂平板上，然后在 37°C 下孵育 24 小时。
使用推荐的清洁方法，在所检查的吸入装置中均未检测到细菌污染。
如果按照制造商的建议清洁吸入室，可以预期成功进行细菌消毒。
