Institute of Microbiology and Biotechnology (IMBT), Vietnam National University, Hanoi, Vietnam.
IHU Méditerranée Infection, Marseille, France.
Int J Syst Evol Microbiol. 2020 Mar;70(3):1758-1768. doi: 10.1099/ijsem.0.003968.
We describe a new multidrug resistant species that was isolated from patients with type 2 diabetes in Vietnam. Strain BD 01 was cultivated in 2017 from a blood sample of a patient suffering from bacteremia. Strain VP 7442 was isolated in 2018 from a pleural fluid sample of a patient who had presented with lung abscess and pleural effusion. Both strains are aerobic, Gram-negative, non-motile and non-spore-forming. The 16S rRNA gene sequences of both strains are 100 % similar and share a highest 16S sequence identity with MRP-15 of 97.42 %. Their predominant fatty acid is iso-C (73.8 % for strain BD 01 and 79.8 % for strain VP 7442). The draft genome sizes of strains BD 01 and VP 7442 are 6 308 408 and 6 308 579 bp, respectively. They are resistant to beta-lactams, aminoglycosides, fluoroquinolones, metronidazole, fosfomycin, vancomycin and macrolides, and exhibit 20 and 18 antimicrobial resistance-related genes, respectively. Using the multiphasic taxonogenomic approach, we propose that strains BD 01 (=CSUR P9622=VTCC 70981) and VP 7442 (=CSUR P9623=VTCC 70982) represent a new species, for which we propose the name sp. nov. Strain BD 01 was chosen as type strain of sp. nov.
我们描述了一种从越南 2 型糖尿病患者中分离出的新的多药耐药种。BD01 株于 2017 年从一名患有菌血症的患者的血液样本中培养。VP7442 株于 2018 年从一名患有肺脓肿和胸腔积液的患者的胸腔积液样本中分离。两株菌均为需氧、革兰氏阴性、非运动和非孢子形成。两株菌的 16S rRNA 基因序列完全相同，与 MRP-15 的最高 16S 序列同一性为 97.42%。它们的主要脂肪酸是异-C（BD01 株为 73.8%，VP7442 株为 79.8%）。BD01 株和 VP7442 株的基因组大小分别为 6308408bp 和 6308579bp。它们对β-内酰胺类、氨基糖苷类、氟喹诺酮类、甲硝唑、磷霉素、万古霉素和大环内酯类抗生素具有耐药性，分别表现出 20 种和 18 种与抗菌药物耐药相关的基因。使用多相分类基因组学方法，我们建议 BD01 株（=CSUR P9622=VTCC 70981）和 VP7442 株（=CSUR P9623=VTCC 70982）代表一个新种，我们建议将其命名为 sp. nov.。BD01 株被选为新种的模式株。
