College of Stomatology, Chongqing Medical University, Chongqing 401147, China.
Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China.
Mater Sci Eng C Mater Biol Appl. 2020 Apr;109:110551. doi: 10.1016/j.msec.2019.110551. Epub 2019 Dec 12.
Accelerated osseointegration is well considered as a critical facilitated factor for titanium scaffold longevity，but immensely limited by bioinert material surfaces and restricted biological responses in vivo. Here, we fabricated three-dimensional printed porous titanium scaffold modified with titanium dioxide (TiO) nanotubes (p-TNTs), for mimicking the hierarchical trabecular bone structure and building local drug delivery system to accelerate osseointegration. The scaffold at macro and micro gradient was manufactured by selective laser melting process with pure titanium powder according to the computer-aided design (CAD), which could be further anodized to construct nano-gradient frameworks and drug-loading districts. Sequentially, 1α,25-Dihydroxyvitamin D3 (VD3) was loaded into anodized TiO nanotubes，thermo-sensitive hydrogel Pluronic F-127 (F-127) was coated and charged as a bio-cap for control release. Biological responses in vitro and in vivo was evaluated, showing significant osteogenesis enhancement of hierarchical structure with local drug delivery. It suggests that a promising bioactive implant system may provide a far-reaching impact on accelerating and enhancing early osseointegration.
加速骨整合被认为是钛支架寿命的关键促进因素，但受到生物惰性材料表面和体内受限的生物反应的极大限制。在这里，我们制造了具有二氧化钛（TiO）纳米管（p-TNTs）的三维打印多孔钛支架，以模拟分级小梁骨结构并构建局部药物输送系统以加速骨整合。支架采用选择性激光熔化工艺根据计算机辅助设计（CAD）用纯钛粉制造，可进一步进行阳极氧化以构建纳米梯度框架和载药区。随后，1α,25-二羟维生素 D3（VD3）被加载到阳极氧化的 TiO 纳米管中，热敏水凝胶泊洛沙姆 F-127（F-127）被涂覆并作为生物帽用于控制释放。体外和体内的生物学反应进行了评估，显示出具有局部药物输送的分级结构的显著成骨增强作用。这表明有前途的生物活性植入系统可能对加速和增强早期骨整合产生深远影响。
