Pulverer G, Ko H L, Beuth J, Roszkowski K, Roszkowski W, Jeljaszewicz J
Hygiene-Institut, Universität Köln.
Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Nov;270(1-2):237-45. doi: 10.1016/s0176-6724(88)80159-7.
Propionibacterium avidum KP 40 cells were mechanically disintegrated in order to obtain the soluble immunostimulatory (antineoplastic) LYSAT. Chemiluminescence measurements of human leukocyte function yielded enhanced activation of the cells after incubation with 2.5 and 5 mg of LYSAT. As compared to non-treated controls, administration of LYSAT to BALB/c-mice (1, 2.5 and 5 mg; intraperitoneally, subcutaneously, intranasal; 7, 4 and 2 days prior to challenge) induced a significant enlargement of the spleen as well as significantly reduced sarcoma L-1 lung colonization 14 days after challenge and evidently enhanced chemiluminescence response of peritoneal macrophages.
为了获得可溶性免疫刺激（抗肿瘤）裂解物，对嗜丙酸杆菌KP 40细胞进行了机械破碎。对人白细胞功能的化学发光测量结果显示，在用2.5毫克和5毫克裂解物孵育后，细胞的活化增强。与未处理的对照组相比，给BALB/c小鼠腹腔内、皮下、鼻内注射裂解物（1毫克、2.5毫克和5毫克；在攻击前7天、4天和2天），可导致脾脏显著肿大，并且在攻击后14天显著减少肉瘤L-1在肺部的定植，同时明显增强腹腔巨噬细胞的化学发光反应。
