Wang Xiaohui, Zhang Wenguang, Hao Yongqing
Key Laboratory of Microbiology & Immunology, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, P.R. China.
School of Public Health, Baotou Medical College, Baotou 014040, P.R. China.
J Vet Sci. 2020 Mar;21(2):e30. doi: 10.4142/jvs.2020.21.e30.
(Mo) is difficult to culture, resulting in many difficulties in related research and application. Since nucleotide metabolism is a basic metabolism affects growth, this study conducted a "point-to-point" comparison of the corresponding growth phases between the Mo NM151 strain and the subsp. (Mmc) PG3 strain. The results showed that the largest difference in nucleotide metabolism was found in the stationary phase. Nucleotide synthesis in PG3 was mostly , while nucleotide synthesis in NM151 was primarily based on salvage synthesis. Compared with PG3, the missing reactions of NM151 referred to the synthesis of deoxythymine monophosphate. We proposed and validated a culture medium with added serine to fill this gap and prolong the stationary phase of NM151. This solved the problem of the fast death of Mo, which is significant for related research and application.
稻瘟病菌（Mo）难以培养，这给相关研究和应用带来了诸多困难。由于核苷酸代谢是影响生长的基础代谢，本研究对稻瘟病菌NM151菌株和稻瘟病菌（Mmc）PG3菌株相应的生长阶段进行了“点对点”比较。结果表明，在稳定期核苷酸代谢的差异最大。PG3中的核苷酸合成大多是从头合成，而NM151中的核苷酸合成主要基于补救合成。与PG3相比，NM151缺失的反应涉及脱氧胸苷单磷酸的合成。我们提出并验证了一种添加丝氨酸的培养基来填补这一空白，并延长NM151的稳定期。这解决了稻瘟病菌快速死亡的问题，对相关研究和应用具有重要意义。
