Institute of Animal Husbandry Research, HeiLongJiang Academy of Agricultural Sciences, Harbin 150086, China.
Key Laboratory of Combine of Planting and Feeding, Ministry of Agriculture of the People's Republic of China, Harbin 150086, China.
Int J Mol Sci. 2020 Mar 22;21(6):2189. doi: 10.3390/ijms21062189.
Two technologies, in vitro culture and exogenous gene introduction, constitute cornerstones of producing transgenic animals. Although in vitro embryo production techniques can bypass the oviduct during early development, such embryos are inferior to their naturally produced counterparts. In addition, preimplantation embryos are resistant to the uptake of exogenous genetic material. These factors restrict the production of transgenic animals. The discovery of extracellular vesicles (EVs) was a milestone in the study of intercellular signal communication. EVs in the oviduct, known as oviductosomes (OVS), are versatile delivery tools during maternal-embryo communication. In this review, we discuss the important roles of OVS in these interactions and the feasibility of using them as tools for transferring exogenous nucleic acids during early development. We hypothesize that further accurate characterization of OVS cargoes and functions will open new horizons for research on maternal-embryo interactions and enhance the production of transgenic animals.
两种技术，体外培养和外源基因导入，构成了生产转基因动物的基石。虽然体外胚胎生产技术可以在早期发育过程中绕过输卵管，但这样的胚胎不如其自然产生的胚胎。此外，胚胎在植入前对外源遗传物质的摄取具有抵抗力。这些因素限制了转基因动物的生产。细胞外囊泡（EVs）的发现是细胞间信号通讯研究的一个里程碑。输卵管中的 EVs，称为卵管体（OVS），是母-胚胎通讯过程中多功能的传递工具。在这篇综述中，我们讨论了 OVS 在这些相互作用中的重要作用，以及将其用作早期发育中外源核酸传递工具的可行性。我们假设对 OVS 货物和功能的进一步精确表征将为母-胚胎相互作用的研究开辟新的视野，并提高转基因动物的生产效率。
