Hernandes Camila, Gueuvoghlanian-Silva Bárbara Yasmin, Monnaka Vitor Ulisses, Ribeiro Natalia Mazini, Pereira Welbert de Oliveira, Podgaec Sérgio
Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
Instituto Israelita de Ensino e Pesquisa Albert Einstein, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
Einstein (Sao Paulo). 2020 Mar 27;18:eAO5294. doi: 10.31744/einstein_journal/2020AO5294. eCollection 2020.
To analyze and compare the expression of Toll-like receptors by regulatory T cells present in the peritoneal fluid of patients with and without endometriosis.
Regulatory T cells were isolated from peritoneal fluid of women with and without endometriosis, collected during surgery, and mRNA was extracted for analysis of Toll-like receptors expression by reverse-transcriptase polymerase chain reaction.
Patients with endometriosis presented regulatory T cells expressing a larger number and variety of Toll-like receptors when compared to regulatory T cells from patients in the Control Group. Toll-like receptor-1 and Toll-like receptor-2 in regulatory T cells were expressed in both groups. All other expressed Toll-like receptors types were only found in regulatory T cells from the Endometriosis Group.
Patients with endometriosis had peritoneal regulatory T cells expressing various Toll-like receptors types.
分析和比较有或无子宫内膜异位症患者腹腔液中调节性T细胞上Toll样受体的表达情况。
从有或无子宫内膜异位症的女性患者手术中收集的腹腔液中分离出调节性T细胞，提取mRNA，通过逆转录聚合酶链反应分析Toll样受体的表达。
与对照组患者的调节性T细胞相比，子宫内膜异位症患者的调节性T细胞表达更多数量和种类的Toll样受体。两组调节性T细胞中均表达Toll样受体-1和Toll样受体-2。所有其他表达的Toll样受体类型仅在子宫内膜异位症组的调节性T细胞中发现。
子宫内膜异位症患者的腹腔调节性T细胞表达多种类型的Toll样受体。
