Institute of Immunology & Infection Research, University of Edinburgh, Ashworth Laboratories, King's Buildings, Charlotte Auerbach Road, Edinburgh, EH9 3FL, UK.
Research Group for Genomic Epidemiology, National Food Institute, Technical University of Denmark, DK-2800, Kongens Lyngby, Denmark.
Commun Biol. 2020 Apr 2;3(1):155. doi: 10.1038/s42003-020-0859-7.
Helminth parasites have been shown to have systemic effects in the host. Using shotgun metagenomic sequencing, we characterise the gut microbiome and resistome of 113 Zimbabwean preschool-aged children (1-5 years). We test the hypothesis that infection with the human helminth parasite, Schistosoma haematobium, is associated with changes in gut microbial and antimicrobial resistance gene abundance/diversity. Here, we show that bacteria phyla Bacteroidetes, Firmicutes, Proteobacteria, and fungi phyla Ascomycota, Microsporidia, Zoopagomycota dominate the microbiome. The abundance of Proteobacteria, Ascomycota, and Basidiomycota differ between schistosome-infected versus uninfected children. Specifically, infection is associated with increases in Pseudomonas, Stenotrophomonas, Derxia, Thalassospira, Aspergillus, Tricholoma, and Periglandula, with a decrease in Azospirillum. We find 262 AMR genes, from 12 functional drug classes, but no association with individual-specific data. To our knowledge, we describe a novel metagenomic dataset of Zimbabwean preschool-aged children, indicating an association between urogenital schistosome infection and changes in the gut microbiome.
寄生虫已被证明在宿主中有全身效应。我们使用 shotgun 宏基因组测序，描述了 113 名津巴布韦学龄前儿童（1-5 岁）的肠道微生物组和抗药性组。我们检验了这样一个假设，即感染人体寄生虫旋毛虫会导致肠道微生物和抗生素耐药性基因丰度/多样性的变化。在这里，我们表明细菌门拟杆菌门、厚壁菌门、变形菌门和真菌门子囊菌门、微孢子虫门、Zoopagomycota 占主导地位。与未感染的儿童相比，感染旋毛虫的儿童肠道中变形菌门、子囊菌门和担子菌门的丰度不同。具体而言，感染与假单胞菌、寡养单胞菌、Derxia、Thalassospira、曲霉属、糙皮侧耳和 Periglandula 的增加有关，而与固氮螺菌的减少有关。我们发现了 262 个 AMR 基因，来自 12 个功能药物类别，但与个体特异性数据没有关联。据我们所知，我们描述了一个津巴布韦学龄前儿童的新型宏基因组数据集，表明尿路血吸虫感染与肠道微生物组的变化之间存在关联。
