Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
Laboratory of Marine Drugs and Biological Products, National Laboratory for Marine Science and Technology, Qingdao 266235, China.
Mar Drugs. 2020 Mar 20;18(3):171. doi: 10.3390/md18030171.
The chemical examination of the marine soft coral sp., collected at the Xisha islands in the South China Sea, resulted in the isolation of four new nardosinane-type sesquiterpenoids, namely clavukoellians G-J (-), and one new aristolane sesquiterpene, namely clavukoellian K (), together with five known compounds, -. The structure elucidation of the isolated natural products was based on various spectroscopic techniques including HRESIMS and NMR, while their absolute configurations were resolved on the basis of comparisons of the ECD spectra with the calculated ECD data. The isolated new compounds - were evaluated for their anti- and pro- angiogenesis activities in a transgenic fluorescent zebrafish (Tg(vegfr2:GFP)) model. Quantitative analysis revealed that compound displayed pro-angiogenesis activity in a PTK787-induced vascular injury zebrafish model at 2.5 μM. Data showed that compound significantly promoted the angiogenesis in a dose-dependent manner.
从中国南海西沙群岛采集的软珊瑚 sp.进行的化学检查，分离得到了四个新的纳多辛烷型倍半萜，分别为clavukoellians G-J (-)，以及一个新的阿里斯托烷倍半萜，即clavukoellian K ( )，以及五个已知化合物， - 。分离出的天然产物的结构阐明基于各种光谱技术，包括 HRESIMS 和 NMR，而它们的绝对构型则是基于与计算 ECD 数据的 ECD 光谱比较来确定的。在转基因荧光斑马鱼 (Tg(vegfr2:GFP))模型中评估了分离出的新化合物 - 的抗血管生成和促血管生成活性。定量分析显示，化合物 2.5 μM 时在 PTK787 诱导的血管损伤斑马鱼模型中表现出促血管生成活性。数据表明，化合物 以剂量依赖性方式显著促进血管生成。
