Llopis-Grimalt Maria Antonia, Arbós Aina, Gil-Mir Maria, Mosur Aleksandra, Kulkarni Prathamesh, Salito Armando, Ramis Joana M, Monjo Marta
Group of Cell Therapy and Tissue Engineering, Department of Fundamental Biology and Health Sciences, Research Institute on Health Sciences (IUNICS), University of the Balearic Islands, 07122 Palma, Spain.
Health Research Institute of the Balearic Islands (IdISBa), 07010 Palma, Spain.
J Clin Med. 2020 Mar 20;9(3):855. doi: 10.3390/jcm9030855.
(1) One strategy to improve the outcome of orthopedic implants is to use porous implants with the addition of a coating with an antibacterial biomolecule. In this study, we aimed to produce and test the biocompatibility, the osteopromotive (both under normal conditions and under a bacterial challenge with lipopolysaccharide (LPS)) and antibacterial activities of a porous Ti-6Al-4V implant coated with the flavonoid quercitrin in vitro. (2) Porous Ti-6Al-4V implants were produced by 3D printing and further functionalized with quercitrin by wet chemistry. Implants were characterized in terms of porosity and mechanical testing, and the coating with quercitrin by fluorescence staining. Implant biocompatibility and bioactivity was tested using MC3T3-E1 preosteoblasts by analyzing cytotoxicity, cell adhesion, osteocalcin production, and alkaline phosphatase (ALP) activity under control and under bacterial challenging conditions using lipopolysaccharide (LPS). Finally, the antibacterial properties of the implants were studied using by measuring bacterial viability and adhesion. (3) Porous implants showed pore size of about 500 µm and a porosity of 52%. The coating was homogeneous over all the 3D surface and did not alter the mechanical properties of the Young modulus. Quercitrin-coated implants showed higher biocompatibility, cell adhesion, and osteocalcin production compared with control implants. Moreover, higher ALP activity was observed for the quercitrin group under both normal and bacterial challenging conditions. Finally, live/dead ratio and adhesion after 4 h of incubation was lower on quercitrin implants compared with the control. (4) Quercitrin-functionalized porous Ti-6Al-4V implants present a great potential as an orthopedic porous implant that decreases bacterial adhesion and viability while promoting bone cell growth and differentiation.
(1) 改善骨科植入物效果的一种策略是使用带有添加了抗菌生物分子涂层的多孔植入物。在本研究中，我们旨在体外制备并测试涂有黄酮类化合物槲皮苷的多孔Ti-6Al-4V植入物的生物相容性、促骨生成能力（在正常条件下以及在脂多糖（LPS）细菌攻击下）和抗菌活性。(2) 多孔Ti-6Al-4V植入物通过3D打印制备，并通过湿化学方法用槲皮苷进一步功能化。对植入物进行孔隙率和力学测试表征，并用荧光染色法对槲皮苷涂层进行表征。使用MC3T3-E1前成骨细胞，通过分析细胞毒性、细胞黏附、骨钙素产生以及在使用脂多糖（LPS）的对照和细菌攻击条件下的碱性磷酸酶（ALP）活性，来测试植入物的生物相容性和生物活性。最后，通过测量细菌活力和黏附来研究植入物的抗菌性能。(3) 多孔植入物的孔径约为500 µm，孔隙率为52%。涂层在整个3D表面上均匀分布，且未改变杨氏模量的力学性能。与对照植入物相比，涂有槲皮苷的植入物表现出更高的生物相容性、细胞黏附性和骨钙素产生。此外，在正常和细菌攻击条件下，槲皮苷组均观察到更高的ALP活性。最后，与对照相比，槲皮苷植入物在孵育4小时后的活/死比率和黏附率更低。(4) 槲皮苷功能化的多孔Ti-6Al-4V植入物作为一种骨科多孔植入物具有巨大潜力，它能降低细菌黏附和活力，同时促进骨细胞生长和分化。
