Construction of a coumarin-based fluorescent probe for accurately visualizing hydrogen sulfide in live cells and zebrafish.

Hydrogen sulfide (HS), an important gas signaling molecule, is a regulator of many physiological processes, and its abnormal levels are closely related to the onset and progression of disease. It is vital to develop methods for specific tracking of HS in clinical diagnosis and treatment. In this study, we designed an ultrasensitive and highly stable coumarin-based fluorescent probe Cou-HS. Through the HS-initiated tandem reaction, Cou-HS successfully achieved highly selective and super-fast detection of HS. Cou-HS was successfully applied to the monitoring of endogenous and exogenous HS at the cellular level and verified the validity of the detection of HS in the LPS-induced zebrafish model. Therefore, Cou-HS might provide new insights into the study of HS-related diseases.