Wang Xianghong, Song Lingjie, Zhao Jie, Zhou Rongtao, Luan Shifang, Huang Yubin, Yin Jinghua, Khan AtherFarooq
State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
J Mater Chem B. 2018 Dec 14;6(46):7710-7718. doi: 10.1039/c8tb01950a. Epub 2018 Nov 6.
A major challenge in antibacterial surface preparation is the elaborated implementation of controlled antibacterial agent delivery on demand, typically at appropriate concentrations and sites, which can be an efficient way to lower bacteria resistance and improve therapy effectiveness. Herein, we present a bacterial hyaluronidase (HAase) and pH dual-responsive antimicrobial surface, which is constructed by sequential layer-by-layer (LbL) assembly of NHS-PEG-NHS (PEG-bis(succinimidyl succinate)) and PEI (polyethylenimine), immobilization of antibiotic vancomycin (Van) by an acid-labile β-carboxylic linker and electrostatic adsorption of HA. The HA upper layer can endow the multilayer surface with excellent biocompatibility under normal physiological conditions. Once bacteria invade, the secreted HAase specifically enzymolyzes the HA layer, and then the bound Van will be released in response to the bacteria-triggered local acidification to eliminate bacteria. Our work provides a versatile strategy in the design of a smart antibacterial surface with controlled antibacterial agent delivery, implying great potential for application in infection-resistant medical devices.
抗菌表面制备中的一个主要挑战是精心实现按需控制抗菌剂的递送，通常是在适当的浓度和部位，这可能是降低细菌耐药性和提高治疗效果的有效方法。在此，我们展示了一种细菌透明质酸酶（HAase）和pH双重响应抗菌表面，它是通过NHS-PEG-NHS（聚乙二醇双琥珀酰亚胺琥珀酸酯）和PEI（聚乙烯亚胺）的逐层（LbL）组装、通过酸不稳定的β-羧基连接子固定抗生素万古霉素（Van）以及HA的静电吸附构建而成。HA上层可以在正常生理条件下赋予多层表面优异的生物相容性。一旦细菌入侵，分泌的HAase会特异性地酶解HA层，然后结合的Van会响应细菌引发的局部酸化而释放，以消除细菌。我们的工作为设计具有可控抗菌剂递送的智能抗菌表面提供了一种通用策略，这意味着在抗感染医疗设备中的应用具有巨大潜力。
