Che Chaoyue, Liu Lin, Wang Xiaodan, Zhang Xu, Luan Shifang, Yin Jinghua, Li Xue, Shi Hengchong
Shandong Provincial Key Laboratory of Fluorine Chemistry and Chemical Materials, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China.
State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.
ACS Biomater Sci Eng. 2020 Mar 9;6(3):1776-1786. doi: 10.1021/acsbiomaterials.0c00069. Epub 2020 Feb 18.
Uncontrolled hemorrhage is the leading factor causing death in military trauma and clinical practice and is usually accompanied with bacterial infection. The fabrication of a functional hemostatic sponge for combating bacteria is of vital importance. Poly(hexamethylene biguanide) hydrochloride (PHMB) has been shown to have a remarkable bactericide effect. However, PHMB after use might have a toxic effect on humans. Herein, a powerful surface-adaptive, on-demand antibacterial hemostatic sodium alginate/gelatin sponge is fabricated by sequential spray-assisted layer-by-layer assembly of PHMB and hyaluronic acid (HA). The HA upper layer could endow the coating surface with better biocompatibility under physiological conditions. Once bacteria invade, the secreted hyaluronidase could degrade the upper HA layer, and then the exposed PHMB layer could kill the bacteria. The coated sponge shows excellent biocompatibility, as negatively charged HA can prevent the exposure of bactericidal PHMB. Moreover, the coated sponge exhibited excellent hemostatic properties and by aggregating and activating blood cells. Our strategy provides a novel approach to the design of surface-adaptive and on-demand bactericidal coatings on the surface of hemostatic sponges, which has the advantage of avoiding infection complications during hemostasis.
失控性出血是军事创伤和临床实践中导致死亡的主要因素，且通常伴有细菌感染。制备一种用于抗菌的功能性止血海绵至关重要。聚（六亚甲基双胍）盐酸盐（PHMB）已被证明具有显著的杀菌效果。然而，使用后的PHMB可能对人体产生毒性作用。在此，通过PHMB和透明质酸（HA）的顺序喷雾辅助逐层组装，制备了一种强大的表面自适应、按需抗菌的海藻酸钠/明胶止血海绵。HA上层可在生理条件下赋予涂层表面更好的生物相容性。一旦细菌入侵，分泌的透明质酸酶可降解上层HA层，然后暴露的PHMB层可杀死细菌。涂层海绵表现出优异的生物相容性，因为带负电荷的HA可防止杀菌性PHMB暴露。此外，涂层海绵通过聚集和激活血细胞表现出优异的止血性能。我们的策略为在止血海绵表面设计表面自适应和按需杀菌涂层提供了一种新方法，具有避免止血过程中感染并发症的优势。
