Chongqing Research Center for Pharmaceutical Engineering, Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, College of Pharmacy, Chongqing Medical University, District of Yuzhong, Chongqing 400016, P. R. China.
J Mater Chem B. 2019 Feb 28;7(8):1246-1257. doi: 10.1039/c8tb03056a. Epub 2019 Jan 31.
In this study, we successfully prepared polydopamine (PD) and hyaluronic acid (HA) coated liquid perfluorocarbon nanoparticles, which integrated the good photothermal conversion effect of PD, the ultrasound imaging properties of the liquid fluorocarbon and active tumor-targeting of HA. Liquid perfluorocarbon nanodroplets significantly improved in terms of their physical stability after being coated with PD and HA as the shell. Photothermal and ultrasound imaging experiments showed that PD could effectively convert near-infrared (NIR) laser energy into heat for photothermal therapy, which could induce a phase change of the liquid perfluorocarbon, thereby generating microbubbles, enhancing ultrasound imaging signals and promoting drug release. In vitro cellular experiments showed that HA on the surface of the nanoparticles could actively target 4T1 breast cancer cells by CD44 receptor-mediated endocytosis. Furthermore, the novel nanosystem has shown effective inhibition of tumor growth by synergistic photothermal-chemotherapy in tumor-bearing mice. In conclusion, the novel nanosystem we prepared in this study is expected to provide a new strategy for the diagnosis and treatment of cancer.
在这项研究中，我们成功制备了聚多巴胺（PD）和透明质酸（HA）包覆的液态全氟碳纳米粒子，整合了 PD 的良好光热转换效果、液态氟碳的超声成像特性以及 HA 的主动肿瘤靶向性。液态全氟碳纳米液滴经 PD 和 HA 包覆后，其物理稳定性显著提高。光热和超声成像实验表明，PD 可有效将近红外（NIR）激光能量转化为热能进行光热治疗，从而诱导液态全氟碳发生相变，产生微泡，增强超声成像信号并促进药物释放。体外细胞实验表明，纳米粒子表面的 HA 可通过 CD44 受体介导的内吞作用主动靶向 4T1 乳腺癌细胞。此外，该新型纳米系统在荷瘤小鼠中通过光热化学协同治疗表现出了有效的肿瘤生长抑制作用。综上所述，我们在本研究中制备的新型纳米系统有望为癌症的诊断和治疗提供新策略。
