Gulbe Gundega, Pilmane Māra, Saulīte Vaira, Doniņa Simona, Jermolajevs Jevgenijs, Peškova Lilija, Valdovska Anda
Riga Stradins University, Institute of Microbiology and Virology, 5 Ratsupites Street, Riga LV-1067, Latvia.
Riga Stradins University, Faculty of Medicine, Institute of Anatomy and Anthropology, Anatomical Theatre, 9 Kronvalda Bulvaris, Riga LV-1010, Latvia.
Mediators Inflamm. 2020 Mar 22;2020:8238029. doi: 10.1155/2020/8238029. eCollection 2020.
The aim of this study was to investigate the effect of intramammary infusions of natural composition GLP 810 with immunomodulating properties on the local nonspecific cellular and humoral immune response in cows with subclinical mastitis. The composition GLP 810 consists of lactic acid, lysozyme, glycopeptides, and 0.9% solution of NaCl. The following parameters were studied: (1) leukocyte differential distribution in milk, (2) expression of cytokines in milk leukocytes, (3) antibacterial activity, and (4) milk quality. Nineteen mammary glands in five lactating cows were infused with 10 mL of GLP 810, and nineteen other glands from five control cows were treated with 10 mL 0.9% NaCl. The results showed that after intramammary administration of the composition GLP 810 three times with 48 h intervals, the following effects on leukocyte populations in milk were observed: (1) an increase in the number of polymorphonuclear leukocytes and lymphocytes and (2) a decrease in the number of macrophages. A reduction in the number of pathogenic bacteria was also detected. The analyses of tumour necrosis factor-alpha, interleukin-10, and beta-defensin-2 revealed that the production of the aforementioned cytokines significantly increased, whereas no significant effects on interleukin-1 and caspase-6 expression in milk leukocytes were recorded. However, there were significant differences between mammary glands with high and low milk somatic cell count. The results suggest that the composition GLP 810 has an immunomodulatory effect on mammary glands and it could be used for improving the immune response in cows with subclinical mastitis during lactation.
本研究的目的是调查具有免疫调节特性的天然成分GLP 810经乳房内灌注对亚临床型乳腺炎奶牛局部非特异性细胞免疫和体液免疫反应的影响。GLP 810成分由乳酸、溶菌酶、糖肽和0.9%的氯化钠溶液组成。研究了以下参数：（1）牛奶中白细胞的差异分布，（2）牛奶白细胞中细胞因子的表达，（3）抗菌活性，以及（4）牛奶质量。对五头泌乳奶牛的19个乳腺灌注10 mL GLP 810，对另外五头对照奶牛的19个乳腺用10 mL 0.9%的氯化钠进行处理。结果显示，在以48小时的间隔对乳房内进行三次GLP 810成分灌注后，观察到对牛奶中白细胞群体有以下影响：（1）多形核白细胞和淋巴细胞数量增加，（2）巨噬细胞数量减少。还检测到病原菌数量减少。对肿瘤坏死因子-α、白细胞介素-10和β-防御素-2的分析表明，上述细胞因子的产生显著增加，而对牛奶白细胞中白细胞介素-1和半胱天冬酶-6的表达未记录到显著影响。然而，在牛奶体细胞计数高和低的乳腺之间存在显著差异。结果表明，GLP 810成分对乳腺具有免疫调节作用，可用于改善泌乳期亚临床型乳腺炎奶牛的免疫反应。
