Department of Biosystems Engineering, College of Agriculture and Life Science, The Institute of Forest Science, Kangwon National University, Chuncheon-24341, Republic of Korea.
Department of Food Science and Biotechnology, Kangwon National University, Chuncheon-24341, Republic of Korea.
Biomed Res Int. 2020 Mar 6;2020:8193971. doi: 10.1155/2020/8193971. eCollection 2020.
Maitake () mushroom has received an enormous amount of attention as a dietary supplement due to its high nutritional values. The particle sizes of mushrooms were monitored by a classifying mill. -Glucans are the bioactive component of the mushroom, and it was revealed through Fourier transform infrared spectroscopy (FTIR), proton and carbon nuclear magnetic resonance (H and C-NMR), matrix-assisted laser desorption/ionization, and time-of-flight (MALDI-TOF) spectrometry. The biocompatibility of particles, as well as induced osteogenesis of hMSCs, was evaluated through WST-1 assay and alizarin staining (ARS) technique, respectively. Notably, enhanced cell viability was noted in the presence of . Significantly improved calcium deposition has observed from hMSCs with suggesting to their mineralization potential. The expression of osteogenic related gene markers was examined in the presence of through real-time polymerase chain reaction (qPCR) technique. The upregulation of osteogenic gene markers in the presence of particles was indicating their superior osteogenic potential. Besides, also activated the secretion of various kinds of proteins from the hMSCs indicating their potential for tissue engineering applications. Enhanced secretion of different immunoglobulins was observed in rat serum in the presence of , further demonstrating their therapeutic nature. Therefore, is effective for enhanced osteogenesis and can be utilized as a natural, edible, and osteogenic agent.
舞茸（Maitake）蘑菇因其高营养价值而作为膳食补充剂受到了极大的关注。通过分类磨对蘑菇颗粒的大小进行了监测。-葡聚糖是蘑菇的生物活性成分，通过傅里叶变换红外光谱（FTIR）、质子和碳核磁共振（H 和 C-NMR）、基质辅助激光解吸/电离和飞行时间（MALDI-TOF）光谱进行了揭示。通过 WST-1 测定法和茜素红染色（ARS）技术分别评估了 颗粒的生物相容性和 hMSCs 的诱导成骨作用。值得注意的是，在存在 的情况下观察到增强的细胞活力。从 hMSCs 中观察到明显改善的钙沉积表明它们具有矿化潜力。通过实时聚合酶链反应（qPCR）技术检查了存在 时与成骨相关的基因标记的表达。在 颗粒存在下上调成骨基因标记表明其具有优越的成骨潜力。此外， 还激活了 hMSCs 分泌各种蛋白质，表明其在组织工程应用中的潜力。在存在 的情况下，在大鼠血清中观察到不同免疫球蛋白的增强分泌，进一步证明了其治疗性质。因此， 可有效促进成骨作用，可作为天然、可食用和具有成骨作用的药物。
