中国临床分离的耐甲氧西林金黄色葡萄球菌的生物膜生成能力。

Biofilm-producing ability of methicillin-resistant Staphylococcus aureus clinically isolated in China.

机构信息

Department of Clinical Laboratory, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China.

Department of Clinical Laboratory, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.

出版信息

BMC Microbiol. 2024 Jul 3;24(1):241. doi: 10.1186/s12866-024-03380-8.

DOI:10.1186/s12866-024-03380-8

PMID:38961344

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11223284/

Abstract

BACKGROUND

Staphylococcus aureus, a commensal bacterium, colonizes the skin and mucous membranes of approximately 30% of the human population. Apart from conventional resistance mechanisms, one of the pathogenic features of S. aureus is its ability to survive in a biofilm state on both biotic and abiotic surfaces. Due to this characteristic, S. aureus is a major cause of human infections, with Methicillin-Resistant Staphylococcus aureus (MRSA) being a significant contributor to both community-acquired and hospital-acquired infections.

RESULTS

Analyzing non-repetitive clinical isolates of MRSA collected from seven provinces and cities in China between 2014 and 2020, it was observed that 53.2% of the MRSA isolates exhibited varying degrees of ability to produce biofilm. The biofilm positivity rate was notably high in MRSA isolates from Guangdong, Jiangxi, and Hubei. The predominant MRSA strains collected in this study were of sequence types ST59, ST5, and ST239, with the biofilm-producing capability mainly distributed among moderate and weak biofilm producers within these ST types. Notably, certain sequence types, such as ST88, exhibited a high prevalence of strong biofilm-producing strains. The study found that SCCmec IV was the predominant type among biofilm-positive MRSA, followed by SCCmec II. Comparing strains with weak and strong biofilm production capabilities, the positive rates of the sdrD and sdrE were higher in strong biofilm producers. The genetic determinants ebp, icaA, icaB, icaC, icaD, icaR, and sdrE were associated with strong biofilm production in MRSA. Additionally, biofilm-negative MRSA isolates showed higher sensitivity rates to cefalotin (94.8%), daptomycin (94.5%), mupirocin (86.5%), teicoplanin (94.5%), fusidic acid (81.0%), and dalbavancin (94.5%) compared to biofilm-positive MRSA isolates. The biofilm positivity rate was consistently above 50% in all collected specimen types.

CONCLUSIONS

MRSA strains with biofilm production capability warrant increased vigilance.

摘要

背景

金黄色葡萄球菌是一种共生细菌，定植于约 30%的人类皮肤和黏膜。除了常规的耐药机制外，金黄色葡萄球菌的致病特征之一是能够在生物膜状态下在生物和非生物表面存活。由于这一特性，金黄色葡萄球菌是人类感染的主要原因，耐甲氧西林金黄色葡萄球菌（MRSA）是社区获得性和医院获得性感染的重要原因。

结果

分析了 2014 年至 2020 年期间在中国七个省市收集的非重复临床 MRSA 分离株，发现 53.2%的 MRSA 分离株表现出不同程度的产生物膜能力。广东、江西和湖北的 MRSA 分离株的生物膜阳性率明显较高。本研究中收集的主要 MRSA 菌株为 ST59、ST5 和 ST239 型，产生物膜能力主要分布在这些 ST 型的中等到弱产生物膜生产者中。值得注意的是，某些 ST 型，如 ST88，表现出高比例的强产生物膜株。研究发现，SCCmec IV 是生物膜阳性 MRSA 中的主要类型，其次是 SCCmec II。比较弱和强产生物膜生产能力的菌株，强产生物膜生产者中 sdrD 和 sdrE 的阳性率更高。ebp、icaA、icaB、icaC、icaD、icaR 和 sdrE 等遗传决定因素与 MRSA 中的强产生物膜有关。此外，与生物膜阴性 MRSA 分离株相比，生物膜阳性 MRSA 分离株对头孢西丁（94.8%）、达托霉素（94.5%）、莫匹罗星（86.5%）、替考拉宁（94.5%）、夫西地酸（81.0%）和达巴万星（94.5%）的敏感性率更高。在所有收集的标本类型中，生物膜阳性率均保持在 50%以上。