Xue Peng, Bao Jingnan, Zhang Lei, Xu Zhigang, Xu Chenjie, Zhang Yilei, Kang Yuejun
Faculty of Materials and Energy, Institute for Clean Energy and Advanced Materials, Southwest University, 2 Tiansheng Road, Beibei, Chongqing 400715, China.
J Mater Chem B. 2016 Jul 21;4(27):4717-4725. doi: 10.1039/c6tb00982d. Epub 2016 Jun 23.
Gene therapy has been developed as an innovative therapeutic modality in the past few decades for treatment of various fatal diseases such as cancer. However, the lack of gene carriers with reliable biosafety and loading capacity is still impeding the practical applications of gene therapy. Moreover, it has become a trend to combine multiple treatment strategies with gene therapy to achieve an enhanced curative effect. Herein, this study proposes the design of a multifunctional nanoplatform for gene delivery and photothermal therapy enhanced by magnetic targeting using functionalized magnetic Prussian blue nanoparticles. Surface modification of magnetic Prussian blue nanoparticles with chitosan and pDNA has been demonstrated to provide excellent colloidal stability and capacity for the magnetic targeting of HeLa cells. These nanocomposites exhibit superparamagnetism, which is remarkable and could potentiality lead to an improvement in their therapeutic effect under a localized magnetic field. The obtained nanoagent is able to generate a significant photothermal effect due to the strong optical absorbance in the near infrared region. Furthermore, superior gene transfection efficiency is achieved under magnetic guidance. In vitro experiments also reveal that this nanoagent has excellent biocompatibility for safe medical applications. This study can provide critical experimental evidence and encourage further investigation of combining photothermal therapy with gene therapy for treatment of various medical conditions.
在过去几十年中，基因治疗已发展成为一种创新的治疗方式，用于治疗各种致命疾病，如癌症。然而，缺乏具有可靠生物安全性和负载能力的基因载体仍然阻碍着基因治疗的实际应用。此外，将多种治疗策略与基因治疗相结合以提高疗效已成为一种趋势。在此，本研究提出设计一种多功能纳米平台，用于基因递送和通过功能化磁性普鲁士蓝纳米颗粒的磁靶向增强光热治疗。用壳聚糖和pDNA对磁性普鲁士蓝纳米颗粒进行表面修饰已被证明可提供优异的胶体稳定性以及对HeLa细胞的磁靶向能力。这些纳米复合材料表现出超顺磁性，这很显著，并且可能会在局部磁场下提高其治疗效果。由于在近红外区域具有强光吸收，所获得的纳米制剂能够产生显著的光热效应。此外，在磁引导下实现了优异的基因转染效率。体外实验还表明，这种纳米制剂具有优异的生物相容性，可用于安全的医学应用。本研究可为光热治疗与基因治疗相结合治疗各种病症提供关键的实验证据，并鼓励进一步研究。
