College of Chemistry and Chemical Engineering, Central South University, Hunan, Changsha 410083, PR China.
College of Chemistry and Chemical Engineering, Central South University, Hunan, Changsha 410083, PR China.
Bioorg Chem. 2024 Feb;143:106981. doi: 10.1016/j.bioorg.2023.106981. Epub 2023 Nov 19.
The combination of chemodynamic therapy and photothermal therapy has a promising application owing to its impressive anti-cancer effects. However, the degradability of the material and the lack of targeting severely limit its further clinical application. Herein, DNAs containing nucleolin aptamer (AS1411) and different bases sequences were used to functionalize PB NPs for the targeted treatment. Compared to prussian blue, DNA-functionalized prussian blue does not reduce the photothermal properties of prussian blue. Moreover, DNA confers DNA-functionalized prussian blue targeting and higher enzymatic activity, thereby achieving a more effective combination of chemodynamic and photothermal treatment. The therapeutic efficacy of this nanoplatform was evaluated in vivo and in vitro experiments, exhibiting that DNA-functionalized prussian blue nanozyme can maximize the precise control of the therapeutic effect, reduce the toxic and side effects caused by non-specific accumulation on other normal cells, and effectively achieve targeted killing of cancer cells. This work demonstrates that DNA-functionalized prussian blue can improve the efficiency of combined tumor treatment and enhance the application value of prussian blue in tumor treatment, which is expected to provide theoretical support for clinical application.
由于其令人印象深刻的抗癌效果，化学动力学治疗和光热治疗的联合具有广阔的应用前景。然而，材料的可降解性和缺乏靶向性严重限制了其进一步的临床应用。在此，我们使用含有核仁素适体（AS1411）和不同碱基序列的 DNA 对 PB NPs 进行功能化，以实现靶向治疗。与普鲁士蓝相比，DNA 功能化的普鲁士蓝不会降低普鲁士蓝的光热性能。此外，DNA 赋予 DNA 功能化的普鲁士蓝靶向性和更高的酶活性，从而实现化学动力学和光热治疗的更有效结合。我们在体内和体外实验中评估了该纳米平台的治疗效果，结果表明，DNA 功能化的普鲁士蓝纳米酶可以最大限度地精确控制治疗效果，减少非特异性积聚在其他正常细胞上引起的毒性和副作用，并有效实现癌细胞的靶向杀伤。这项工作表明，DNA 功能化的普鲁士蓝可以提高联合肿瘤治疗的效率，并增强普鲁士蓝在肿瘤治疗中的应用价值，有望为临床应用提供理论支持。
