Murali Ragothaman, Thanikaivelan Palanisamy, Cheirmadurai Kalirajan
Advanced Materials Laboratory, Central Leather Research Institute (Council of Scientific and Industrial Research), Adyar, Chennai 600020, India.
J Mater Chem B. 2016 Sep 21;4(35):5850-5862. doi: 10.1039/c6tb01221c. Epub 2016 Aug 17.
The development of hybrid scaffolds mimicking the extracellular matrix with bioactive factors has great potential to regenerate tissues in tissue engineering and wound-healing applications. Herein, poly(dialdehyde) gum acacia was synthesized by the selective oxidation of gum acacia and was blended with collagen and melatonin to fabricate biomimetic hybrid scaffolds. The inclusion of poly(dialdehyde) gum acacia improved the stability of collagen and immobilized the melatonin in the hybrid scaffolds. The prepared hybrid scaffolds showed significant biocompatibility when cultured with the Swiss 3T6 mouse fibroblast cell lines in vitro. When subjected to open excisional skin wounds in Wistar albino rats in vivo, the collagen-poly(dialdehyde) gum acacia-melatonin hybrid scaffolds accelerated re-epithelialization and collagen deposition. The results indicate that the melatonin-immobilized hybrid scaffold promotes rapid tissue regeneration and wound repair owing to its antioxidant and anti-inflammatory properties, thereby demonstrating its potential for application in burns and chronic wounds.
开发具有生物活性因子、模仿细胞外基质的混合支架在组织工程和伤口愈合应用中具有巨大的组织再生潜力。在此，通过对阿拉伯胶进行选择性氧化合成了聚（二醛）阿拉伯胶，并将其与胶原蛋白和褪黑素混合以制备仿生混合支架。聚（二醛）阿拉伯胶的加入提高了胶原蛋白的稳定性，并将褪黑素固定在混合支架中。制备的混合支架在体外与瑞士3T6小鼠成纤维细胞系培养时表现出显著的生物相容性。当在体内对Wistar白化大鼠进行开放性切除皮肤伤口实验时，胶原蛋白 - 聚（二醛）阿拉伯胶 - 褪黑素混合支架加速了上皮再形成和胶原蛋白沉积。结果表明，固定有褪黑素的混合支架由于其抗氧化和抗炎特性而促进快速组织再生和伤口修复，从而证明了其在烧伤和慢性伤口中的应用潜力。
