Chin Jiah Shin, Madden Leigh E, Phillips Anthony R J, Chew Sing Yian, Becker David L
Nanyang Institute of Health Technologies, Interdisciplinary Graduate School, Nanyang Technological University, Singapore 639798, Singapore.
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637459, Singapore.
Bioengineering (Basel). 2022 Jul 18;9(7):324. doi: 10.3390/bioengineering9070324.
Scaffolds can promote the healing of burns and chronic skin wounds but to date have suffered from issues with achieving full skin integration. Here, we characterise the wound response by both tissue integration and re-epithelialization to a scaffold using wet electrospinning to fabricate 3D fibrous structures. Two scaffold materials were investigated: poly(ε-caprolactone) (PCL) and PCL + 20% rat tail type 1 collagen (PCL/Coll). We assessed re-epithelisation, inflammatory responses, angiogenesis and the formation of new extracellular matrix (ECM) within the scaffolds in rat acute wounds. The 3D PCL/Coll scaffolds impeded wound re-epithelisation, inducing a thickening of wound-edge epidermis as opposed to a thin tongue of migratory keratinocytes as seen when 3D PCL scaffolds were implanted in the wounds. A significant inflammatory response was observed with 3D PCL/Coll scaffolds but not with 3D PCL scaffolds. Enhanced fibroblast migration and angiogenesis into 3D PCL scaffolds was observed with a significant deposition of new ECM. We observed that this deposition of new ECM within the scaffold was key to enabling re-epithelialization over the scaffold. Such scaffolds provide a biocompatible environment for cell integration to lay down new ECM and encourage re-epithelisation over the implanted scaffold.
支架可以促进烧伤和慢性皮肤伤口的愈合，但迄今为止，在实现完全皮肤整合方面仍存在问题。在此，我们通过组织整合和再上皮化来表征伤口对一种使用湿法静电纺丝制造三维纤维结构的支架的反应。研究了两种支架材料：聚（ε-己内酯）（PCL）和PCL + 20%大鼠尾I型胶原（PCL/Coll）。我们评估了大鼠急性伤口中支架内的再上皮化、炎症反应、血管生成以及新细胞外基质（ECM）的形成。三维PCL/Coll支架阻碍了伤口再上皮化，导致伤口边缘表皮增厚，这与植入三维PCL支架时观察到的迁移角质形成细胞的薄舌状不同。在三维PCL/Coll支架中观察到显著的炎症反应，但在三维PCL支架中未观察到。观察到成纤维细胞向三维PCL支架的迁移和血管生成增强，并有新ECM的显著沉积。我们观察到支架内新ECM的这种沉积是使支架上实现再上皮化的关键。这种支架为细胞整合提供了一个生物相容性环境，以沉积新的ECM并促进植入支架上的再上皮化。
