Li Jingchao, Chen Ying, Yang Yingjun, Kawazoe Naoki, Chen Guoping
International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
J Mater Chem B. 2017 Feb 21;5(7):1353-1362. doi: 10.1039/c6tb03276a. Epub 2017 Jan 31.
Sub-10 nm gold nanoparticles (Au NPs) have attracted extensive attention for different biomedical applications because of their small size. However, the influence of small NPs on the differentiation of human bone marrow-derived mesenchymal stem cells (hMSCs) is unclear. In this study, 4 nm small Au NPs (Au4-mPEG NPs) were synthesized to investigate their influence on the osteogenic and adipogenic differentiation of hMSCs and 40-nm counterparts (Au40-mPEG NPs) were used for comparison. Different from Au40-mPEG NPs, Au4-mPEG NPs reduced the ALP activity, calcium deposition and osteogenic marker gene expression, while increased the oil droplet formation and adipogenic marker gene expression in hMSCs. The mechanism study indicated that Au4-mPEG NP treatment did not affect the cellular mechanical property significantly but induced a high level of reactive oxygen species (ROS). The results provide some insights into the influence of sub-10 nm Au NPs on the stem cell functions and applications of these small particles for tissue engineering.
小于10纳米的金纳米颗粒（Au NPs）因其尺寸小而在不同的生物医学应用中引起了广泛关注。然而，小尺寸纳米颗粒对人骨髓间充质干细胞（hMSCs）分化的影响尚不清楚。在本研究中，合成了4纳米的小金纳米颗粒（Au4-mPEG NPs）以研究其对hMSCs成骨和成脂分化的影响，并使用40纳米的对应物（Au40-mPEG NPs）进行比较。与Au40-mPEG NPs不同，Au4-mPEG NPs降低了hMSCs中的碱性磷酸酶（ALP）活性、钙沉积和成骨标记基因表达，同时增加了油滴形成和成脂标记基因表达。机制研究表明，Au4-mPEG NP处理对细胞力学性能影响不显著，但诱导了高水平的活性氧（ROS）。这些结果为小于10纳米的Au NPs对干细胞功能的影响以及这些小颗粒在组织工程中的应用提供了一些见解。
