Xie Qin, Ma Liying, Tan Peng, Deng Wentao, Huang Chao, Liu Deming, Lin Wanhuang, Su Yi
Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China.
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
Plants (Basel). 2020 Apr 8;9(4):470. doi: 10.3390/plants9040470.
Potassium is an important essential element for plant growth and development. Long-term potassium deprivation can lead to a severe deficiency phenotype in plants. Interestingly, is a plant with an unusually high potassium content and can grow well and complete its lifecycle even in severely potassium deficient soil. In this study, we found that its stems and leaves were the main tissues for high potassium accumulation, and showed a strong ability of K absorption in roots and a large capability of potassium accumulation in shoots. Analysis of plant growth and physiological characteristics indicated that had an adaptability in a wide range of external potassium levels. To reveal the mechanism of K uptake and transport in the potassium-hyperaccumulator plant , K uptake-/transport-related genes were screened by transcriptome sequencing, and their expression profiles were compared between K starved plants and normal cultured plants. Eighteen members of HAK/KT/KUPs, ten members of AKTs, and one member of HKT were identified in . Among them, six , and two and showed significantly different expression. These transporters might be coordinatively involved in K uptake/transport in and lead to high potassium accumulation in plant tissues. In addition, significantly changed expression of some ABC transporters indicated that ABC transporters might be important for K uptake and transport in under low K concentrations.
钾是植物生长发育所必需的重要元素。长期缺钾会导致植物出现严重的缺素表型。有趣的是，[植物名称]是一种钾含量异常高的植物，即使在严重缺钾的土壤中也能生长良好并完成其生命周期。在本研究中，我们发现其茎和叶是高钾积累的主要组织，[植物名称]在根中表现出很强的钾吸收能力，在地上部具有很大的钾积累能力。对植物生长和生理特性的分析表明，[植物名称]在广泛的外部钾水平范围内具有适应性。为了揭示钾超积累植物[植物名称]中钾吸收和转运的机制，通过转录组测序筛选了与钾吸收/转运相关的基因，并比较了缺钾植物和正常培养植物之间它们的表达谱。在[植物名称]中鉴定出18个HAK/KT/KUP成员、10个AKT成员和1个HKT成员。其中，6个[基因名称]、2个[基因名称]和[基因名称]表现出显著不同的表达。这些转运蛋白可能协同参与[植物名称]中的钾吸收/转运，并导致植物组织中高钾积累。此外，一些ABC转运蛋白的表达发生显著变化，表明ABC转运蛋白在低钾浓度下对[植物名称]中的钾吸收和转运可能很重要。
