Department of Virus and Microbiological Special Diagnostics, Statens Serum Institut, Copenhagen, Denmark.
Euro Surveill. 2020 Apr;25(14). doi: 10.2807/1560-7917.ES.2020.25.14.2000398.
The World Health Organization has declared COVID-19 caused by the newly discovered SARS-CoV-2 a pandemic. Due to growing demand for reagents and/or kits to extract SARS-CoV-2 RNA for subsequent RT-qPCR diagnostics, there is a worldwide risk of shortages. With a detection sensitivity of 97.4% (95% CI: 86.2-99.9%), we describe a simple, fast, alternative workflow for molecular detection of SARS-CoV-2, where samples are simply heat-processed for 5 min at 98 °C before a commonly-used RT-qPCR procedure.
世界卫生组织已宣布由新型 SARS-CoV-2 引起的 COVID-19 为大流行。由于对提取 SARS-CoV-2 RNA 的试剂和/或试剂盒的需求不断增加，以便随后进行 RT-qPCR 诊断，因此存在全球范围内短缺的风险。我们描述了一种简单、快速的分子检测 SARS-CoV-2 的替代工作流程，该方法的检测灵敏度为 97.4%（95%CI：86.2-99.9%），只需将样品在 98°C 下简单热处理 5 分钟，然后再进行常用的 RT-qPCR 程序即可。
