Lim Yun Kyong, Yoo So Young, Jang Young Yoon, Lee Byoung Cheon, Lee Dae Sung, Kook Joong-Ki
1Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju, 61452 Republic of Korea.
Medi Bio Lab Co., Ltd., Seoul, 61452 Republic of Korea.
Food Sci Biotechnol. 2019 Nov 1;29(4):539-548. doi: 10.1007/s10068-019-00697-3. eCollection 2020 Apr.
The purpose of this study was to determine the anti-inflammatory and in vitro bone formation effects of L. (mangosteen) and propolis extracts. Immortalized human gingival fibroblasts (hTERT-hNOF) cells were treated with KCOM 2804 lipopolysaccharide followed by treatment with mangosteen and propolis extract alone or in combination. Expression levels of inflammatory cytokines were evaluated by enzyme-linked immunosorbent assay. Effect of mangosteen and/or propolis extracts on mineralization of MG-63 cells was evaluated by alkaline phosphatase activity and alizarin red S staining. Group mangosteen extract complex 1:34 (1 µg/ml mangosteen extract and 34 µg/ml propolis extract) significantly reduced expression levels of IL-6, IL-8, and PGE. It had higher than other groups in vitro bone formation effect on MG63 cells. These results suggest that mangosteen and propolis extract complex could be used in the prevention and treatment of periodontal disease.
本研究的目的是确定山竹和蜂胶提取物的抗炎及体外成骨作用。将永生化人牙龈成纤维细胞（hTERT-hNOF）用KCOM 2804脂多糖处理，随后单独或联合使用山竹和蜂胶提取物进行处理。通过酶联免疫吸附测定法评估炎性细胞因子的表达水平。通过碱性磷酸酶活性和茜素红S染色评估山竹和/或蜂胶提取物对MG-63细胞矿化的影响。山竹提取物复合物组1:34（1μg/ml山竹提取物和34μg/ml蜂胶提取物）显著降低了IL-6、IL-8和PGE的表达水平。其对MG63细胞的体外成骨作用高于其他组。这些结果表明，山竹和蜂胶提取物复合物可用于预防和治疗牙周疾病。
