Huang Kuo, Jiang Qipeng, Liu Liehua, Zhang Shuting, Liu Chaoli, Chen Haitao, Ding Wei, Zhang Yongqiang
College of Plant Protection, Southwest University, Chongqing, 400715, China.
Chongqing Institute of Tobacco Science, Chongqing, 400715, People's Republic of China.
AMB Express. 2020 Apr 15;10(1):72. doi: 10.1186/s13568-020-01006-6.
Root-knot nematode (RKN) disease is a soil-borne disease. However, most studies on RKN have focused on the screening of agents and the cultivation of resistant varieties, and reports on the interaction of RKNs with soil microorganisms are few. In this study, we performed Illumina high-throughput sequencing to analyze diseased and healthy soil and the microbial-community changes in rhizosphere soil after microbial treatment (Pseudomonas flurescens, Bacillus subtilis, Paecolomyces lilacinus). Results showed significant differences in the bacterial community richness and diversity between diseased and healthy soil and the presence of different microbial species. After treatment, the richness and diversity of microbial communities in soil, as well as the number and incidence of second-stage juvenile of RKNs, decreased. Through linear discriminant analysis effect size, Pearson correlation, and Venn diagram analysis, we screened five genera that were closely related to disease occurrence, among which Pseudomonas was most related to disease inhibition. Our results suggested that the occurrence of tobacco RKN was related to changes in soil microbial communities, and that the interactions among Pseudomonas, Bryobacter, Variibacter, Coniochaeta, and Metarhizium affected the health of rhizosphere soil.
根结线虫（RKN）病是一种土传病害。然而，大多数关于根结线虫的研究都集中在药剂筛选和抗性品种培育上，关于根结线虫与土壤微生物相互作用的报道较少。在本研究中，我们进行了Illumina高通量测序，以分析患病土壤和健康土壤以及微生物处理（荧光假单胞菌、枯草芽孢杆菌、淡紫拟青霉）后根际土壤中的微生物群落变化。结果表明，患病土壤和健康土壤之间的细菌群落丰富度和多样性存在显著差异，且存在不同的微生物种类。处理后，土壤中微生物群落的丰富度和多样性以及根结线虫二龄幼虫的数量和发生率均有所下降。通过线性判别分析效应大小、Pearson相关性和Venn图分析，我们筛选出了五个与病害发生密切相关的属，其中假单胞菌与病害抑制关系最为密切。我们的结果表明，烟草根结线虫的发生与土壤微生物群落的变化有关，假单胞菌、Bryobacter、Variibacter、Coniochaeta和绿僵菌之间的相互作用影响了根际土壤的健康状况。
