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便携式测序作为保护和生物多样性研究中的教学工具。

Portable sequencing as a teaching tool in conservation and biodiversity research.

机构信息

Field Projects International, Saint Louis, Missouri, United States of America.

Population Sustainability, San Diego Zoo Global, San Diego, California, United States of America.

出版信息

PLoS Biol. 2020 Apr 16;18(4):e3000667. doi: 10.1371/journal.pbio.3000667. eCollection 2020 Apr.

Abstract

As biodiversity loss continues to accelerate, there is a critical need for education and biomonitoring across the globe. Portable technologies allow for in situ molecular biodiversity monitoring that has been historically out of reach for many researchers in habitat nations. In the realm of education, portable tools such as DNA sequencers facilitate in situ hands-on training in real-time sequencing and interpretation techniques. Here, we provide step-by-step protocols as a blueprint for a terrestrial conservation genetics field training program that uses low-cost, portable devices to conduct genomics-based training directly in biodiverse habitat countries.

摘要

随着生物多样性的持续丧失,全球范围内对教育和生物监测的需求非常迫切。便携式技术可实现原位分子生物多样性监测,而这在历史上是许多栖息地国家的研究人员无法企及的。在教育领域,DNA 测序仪等便携式工具可实现实时测序和解释技术的现场实践培训。在这里,我们提供了分步协议,作为一个使用低成本、便携式设备直接在生物多样性栖息地国家进行基于基因组学培训的陆地保护遗传学实地培训计划的蓝图。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a3c/7188297/1a9e79e3ca58/pbio.3000667.g001.jpg

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