Epithelial Biology Center.
Department of Pathology, Microbiology, and Immunology.
Biomed Res. 2020;41(2):113-118. doi: 10.2220/biomedres.41.113.
Clinical interest into the function of tuft cells in human intestine has increased in recent years. However, no quantitative study has examined intestinal tuft cells in pathological specimens from patients. This study quantified tuft cell density by using a recently identified marker, specific for tyrosine phosphorylation (pY1798) of girdin (also known as CCDC88A or GIV) in the duodenum of pediatric patients. Deidentified sections with pathological diagnosis of acute duodenitis, ulcer, or celiac disease, and age-matched normal control were analyzed under double-blind conditions. Immunostaining for pY1798-girdin demonstrated the distinct shape of tuft cells with and filopodia-like basolateral membrane structure and a small apical area, which densely expressed gamma-actin. As compared to normal tissues, the specimens diagnosed as celiac disease and duodenal ulcer had significantly fewer tuft cell numbers. In contrast, acute duodenitis showed varied population of tuft cells. The mucosa with severe inflammation showed lower tuft cell numbers than the specimens with none to mild inflammation. These results suggest that loss of tuft cells may be involved in prolonged inflammation in the duodenal mucosa and disrupted mucosal integrity. pY1798-girdin and gamma-actin are useful markers for investigating the distribution and morphologies of human intestinal tuft cells under healthy and pathological conditions.
近年来，人们对肠簇细胞功能的临床研究兴趣日益增加。然而，尚无研究定量检测过病理标本中的肠簇细胞。本研究采用一种新鉴定的标志物，即 girdin（也称为 CCDC88A 或 GIV）酪氨酸磷酸化（pY1798），对儿科患者十二指肠中的簇细胞密度进行了定量研究。在双盲条件下，对经病理诊断为急性十二指肠炎、溃疡或乳糜泻、并与年龄匹配的正常对照的无身份识别切片进行了分析。免疫组化染色显示 pY1798-girdin 标记的簇细胞形态独特，具有丝状伪足样的基底外侧膜结构和较小的顶区，其γ-肌动蛋白表达密集。与正常组织相比，乳糜泻和十二指肠溃疡的标本簇细胞数量明显减少。相比之下，急性十二指肠炎的簇细胞数量存在差异。严重炎症的黏膜簇细胞数量低于无炎症或轻度炎症的标本。这些结果表明，簇细胞的丢失可能与十二指肠黏膜的长期炎症和黏膜完整性的破坏有关。pY1798-girdin 和 γ-肌动蛋白是研究健康和病理条件下人类肠簇细胞分布和形态的有用标志物。
