García-Sánchez Andrés, Gámez-Nava Jorge Iván, Díaz-de la Cruz Elodia Nataly, Cardona-Muñoz Ernesto Germán, Becerra-Alvarado Itzel Nayar, Aceves-Aceves Javier Alejandro, Sánchez-Rodríguez Esther Nérida, Miranda-Díaz Alejandra Guillermina
Department of Physiology, University Health Sciences Centre, University of Guadalajara, Guadalajara, Jalisco, Mexico.
Diabetes Metab Syndr Obes. 2020 Apr 8;13:1077-1087. doi: 10.2147/DMSO.S245494. eCollection 2020.
The increase of visceral abdominal fat (VAF) and oxidative stress (OS) are independent predictors for cardiovascular risk. This study aimed to determine the association of VAF with proinflammatory cytokines, oxidants, antioxidants, and oxidative damage to DNA in subjects with normal weight, overweight, and obesity.
A cross-sectional study that included 21 men and 71 women who attended for a medical check-up was conducted. Dual-energy X-ray absorptiometry (DXA) was used to measure the VAF volume. ELISA and colorimetric techniques were used for chemical analysis.
Low activity of superoxide dismutase (SOD) was found in overweight and obese subjects compared to the normal weight group (=0.005). In contrast, the activity of glutathione peroxidase (GPx) was higher in the overweight and obesity groups compared to the normal weight subjects (=0.017). The total antioxidant capacity (TAC) was also increased in the overweight group compared to the normal weight group (=0.04). According to the volume of VAF, the levels of tumor necrosis factor alfa and interleukin 6 showed no differences between subjects with normal and high VAF. Subjects with high VAF show higher levels of 8-isoprostans compared to normal VAF group (=0.039). Less concentration of 8-oxoguanine-DNA-N-glycosylase-1 (hOGG1) was found in the high VAF group (=0.032) compared to the normal VAF subjects. VAF was positively correlated with lipoperoxides (LPO) (r=0.27, <0.05) and 8-isoprostanes (r=0.25, <0.05). We also found correlations between oxidative stress markers and anthropometric ratios for intra-abdominal fat. The waist-hip ratio was positively correlated with LPO (r=0.30, <0.05) and TAC (r=0.24, <0.05).
These findings suggest that the predominantly oxidative damage associated with VAF in overweight or obesity is lipoperoxidation and oxidative DNA damage. Alterations in endogenous antioxidant defenses may not be linked to the amount of VAF.
内脏腹部脂肪（VAF）增加和氧化应激（OS）是心血管风险的独立预测因素。本研究旨在确定正常体重、超重和肥胖受试者中VAF与促炎细胞因子、氧化剂、抗氧化剂以及DNA氧化损伤之间的关联。
进行了一项横断面研究，纳入了21名男性和71名前来进行体检的女性。采用双能X线吸收法（DXA）测量VAF体积。采用酶联免疫吸附测定（ELISA）和比色技术进行化学分析。
与正常体重组相比，超重和肥胖受试者中超氧化物歧化酶（SOD）活性较低（P=0.005）。相反，与正常体重受试者相比，超重和肥胖组中谷胱甘肽过氧化物酶（GPx）活性较高（P=0.017）。与正常体重组相比，超重组的总抗氧化能力（TAC）也有所增加（P=0.04）。根据VAF体积，肿瘤坏死因子α和白细胞介素6水平在正常VAF和高VAF受试者之间无差异。与正常VAF组相比，高VAF受试者的8-异前列腺素水平更高（P=0.039）。与正常VAF受试者相比，高VAF组中8-氧代鸟嘌呤-DNA-N-糖基化酶-1（hOGG1）的浓度更低（P=0.032）。VAF与脂质过氧化物（LPO）呈正相关（r=0.27，P<0.05），与8-异前列腺素呈正相关（r=0.25，P<0.05）。我们还发现氧化应激标志物与腹部脂肪的人体测量比值之间存在相关性。腰臀比与LPO呈正相关（r=0.30，P<0.05），与TAC呈正相关（r=0.24，P<0.05）。
这些发现表明，超重或肥胖中与VAF相关的主要氧化损伤是脂质过氧化和DNA氧化损伤。内源性抗氧化防御的改变可能与VAF的量无关。
