Wu Xin, Wang Kun, Cui Shuai, Wu Shengbing, Zhu Guoqi, Zhou Meiqi
Graduate School, Anhui University of Chinese Medicine, Hefei 230038, China.
Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei 230038, China.
Evid Based Complement Alternat Med. 2020 Mar 31;2020:4298657. doi: 10.1155/2020/4298657. eCollection 2020.
The locus coeruleus (LC) is closely linked with cardiovascular disease. However, whether it mediates the alleviating effect of electroacupuncture (EA) on acute myocardial ischemia (AMI) remains unclear. A rat model of myocardial ischemia was established through occlusion of the left anterior descending coronary artery. Multichannel recording and other techniques were used to assess neurons in the LC, norepinephrine (NE) and dopamine (DA) levels in central and myocardial tissue, serum levels of inflammatory factors, and cardiac function. After induction of AMI, LC neuron activity increased and the central NE concentrations increased, while those of DA decreased. Moreover, the serum levels of high-sensitivity C-reactive protein (hs-CRP) increased, whereas those of interleukin-10 (IL-10) decreased. However, these effects were reversed by EA. Additionally, LC lesioning affected NE and DA levels in myocardial tissue and weakened the antimyocardial ischemic effect of EA. Collectively, our results indicated that LC is closely related to AMI and plays an important role in the antimyocardial ischemic effect of EA. This mechanism may be related to inhibition of LC neuron activity by EA, which inhibits the release of large amounts of hs-CRP and promotes that of IL-10 in the serum. Besides, after LC lesioning, EA may improve cardiac function by inhibiting the release of large amounts of NE and promoting the release of DA in myocardial tissue.
蓝斑（LC）与心血管疾病密切相关。然而，其是否介导电针（EA）对急性心肌缺血（AMI）的缓解作用仍不清楚。通过结扎左冠状动脉前降支建立大鼠心肌缺血模型。采用多通道记录等技术评估LC中的神经元、中枢和心肌组织中的去甲肾上腺素（NE）和多巴胺（DA）水平、炎症因子血清水平及心功能。诱导AMI后，LC神经元活动增加，中枢NE浓度升高，而DA浓度降低。此外，高敏C反应蛋白（hs-CRP）血清水平升高，而白细胞介素-10（IL-10）血清水平降低。然而，这些效应被EA逆转。此外，损毁LC影响心肌组织中的NE和DA水平，并削弱了EA的抗心肌缺血作用。总体而言，我们的结果表明LC与AMI密切相关，且在EA的抗心肌缺血作用中起重要作用。该机制可能与EA抑制LC神经元活动有关，EA抑制血清中大量hs-CRP的释放并促进IL-10的释放。此外，损毁LC后，EA可能通过抑制心肌组织中大量NE的释放并促进DA的释放来改善心功能。
