来自中国一只蝙蝠的病毒宏基因组学中的多种单链DNA病毒。

Diverse single-stranded DNA viruses from viral metagenomics on a bat in China.

作者信息

Kane Yakhouba, Chen Jinping, Li Linmiao, Descorps-Declère Stéphane, Wong Gary, Berthet Nicolas

机构信息

Viral Hemorrhagic Fevers Research Unit, CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, China.

University of Chinese Academy of Sciences, Beijing 100049, China.

出版信息

Heliyon. 2023 Jul 17;9(8):e18270. doi: 10.1016/j.heliyon.2023.e18270. eCollection 2023 Aug.

DOI:10.1016/j.heliyon.2023.e18270

PMID:37520955

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10374907/

Abstract

Bats serve as reservoirs for many emerging viruses. Cressdnaviruses can infect a wide range of animals, including agricultural species, such as pigs, in which porcine circoviruses cause severe gastroenteritis. New cressdnaviruses have also attracted considerable attention recently, due to their involvement with infectious diseases. However, little is known about their host range and many cressdnaviruses remain poorly characterized. We identified and characterized 11 contigs consisting of previously unknown cressdnaviruses from a rectal swab sample of a bat collected in Yunnan Province, China, in 2011. Full genomes of two cressdnaviruses (OQ267680, 2069 nt; OQ351951, 2382 nt), and a nearly complete genome for a third (OQ267683, 2361 nt) were obtained. Phylogenetic analyses and the characteristics of these viral genomes suggest a high degree of ssDNA virus diversity. These results shed light on cressdnavirus diversity and the probable role of bats as their hosts.

摘要

蝙蝠是许多新兴病毒的宿主。环状单链DNA病毒可感染多种动物，包括农业养殖物种，如猪，猪圆环病毒可在猪体内引发严重肠胃炎。新型环状单链DNA病毒因其与传染病的关联，近来也备受关注。然而，人们对其宿主范围知之甚少，许多环状单链DNA病毒的特征仍不清楚。我们从2011年在中国云南省采集的一只蝙蝠的直肠拭子样本中，鉴定并表征了11个由此前未知的环状单链DNA病毒组成的重叠群。获得了两种环状单链DNA病毒的全基因组（OQ267680，2069个核苷酸；OQ351951，2382个核苷酸），以及第三种病毒的近乎完整的基因组（OQ267683，2361个核苷酸）。系统发育分析以及这些病毒基因组的特征表明，单链DNA病毒具有高度多样性。这些结果揭示了环状单链DNA病毒的多样性以及蝙蝠作为其宿主的可能作用。