College of Forestry, Northeast Forestry University, Harbin, China.
College of Agricultural and Biological Engineering, Heze University, Heze, China.
FEBS Open Bio. 2020 Jun;10(6):1171-1179. doi: 10.1002/2211-5463.12871. Epub 2020 May 22.
Pinewood nematode (PWN; Bursaphelenchus xylophilus) is a devastating invasive species that is expanding into colder regions. Here, we investigated the molecular mechanisms underlying low-temperature resistance of PWN. We identified differentially expressed genes enriched under low temperature in previously published transcriptome data using the Kyoto Encyclopedia of Genes and Genomes. Quantitative real-time PCR was used to further validate the transcript level changes of three known cytochrome P450 genes under low temperature. RNA interference was used to validate the low-temperature resistance function of three cytochrome P450 genes from PWN. We report that differentially expressed genes were significantly enriched in two cytochrome P450-related pathways under low-temperature treatment. Heatmap visualization of transcript levels of cytochrome P450-related genes revealed widely different transcript patterns between PWNs treated under low and regular temperatures. Transcript levels of three cytochrome P450 genes from PWNs were elevated at low temperature, and knockdown of these genes decreased the survival rates of PWNs under low temperature. In summary, these findings suggest that cytochrome P450 metabolism plays a critical role in the low-temperature resistance mechanism of PWN.
松材线虫（PWN；Bursaphelenchus xylophilus）是一种具有破坏性的入侵物种，正在向较冷的地区扩张。在这里，我们研究了 PWN 低温抗性的分子机制。我们使用京都基因与基因组百科全书（KEGG），从之前发表的转录组数据中鉴定了低温下差异表达的基因。定量实时 PCR 进一步验证了三种已知细胞色素 P450 基因在低温下的转录水平变化。RNA 干扰用于验证三种来自 PWN 的细胞色素 P450 基因的低温抗性功能。我们报告说，在低温处理下，差异表达的基因在两个细胞色素 P450 相关途径中显著富集。细胞色素 P450 相关基因转录水平的热图可视化显示，低温和常规温度处理的 PWN 之间的转录模式有很大差异。PWN 中的三种细胞色素 P450 基因的转录水平在低温下升高，这些基因的敲低降低了 PWN 在低温下的存活率。总之，这些发现表明细胞色素 P450 代谢在 PWN 的低温抗性机制中起关键作用。
