College of Forestry, Northeast Forestry University, Harbin 150040, China.
College of Management, Harbin University of Commerce, Harbin 150028, China.
Int J Mol Sci. 2017 Nov 3;18(11):2320. doi: 10.3390/ijms18112320.
The causal agent of pine wilt disease, pine wood nematode (PWN) (), revealed extended lifespan at low temperature. To discover the molecular mechanism of this phenomenon, we attempted to study the molecular characterization, transcript abundance, and functions of three genes of the cyclic guanosine monophosphate (cGMP) pathway from . Three cGMP pathway genes were identified from . Bioinformatic software was utilized to analyze the characteristics of the three putative proteins. Function of the three genes in cold tolerance was studied with RNA interference (RNAi). The results showed that the deduced protein of Bx-DAF-11 has an adenylate and guanylate cyclase catalytic domain, indicating an ability to bind to extracellular ligands and synthesizing cGMP. Both Bx-TAX-2 and Bx-TAX-4 have cyclic nucleotide-binding domains and ion transport protein domains, illustrating that they are cGMP-gated ion channels. The transcript level of , , and increased at low temperature. The survival rates of three gene silenced revealed a significant decrease at low temperature. This study illustrated that the cGMP pathway plays a key role in low-temperature-induced lifespan extension in .
松材线虫是松材线虫病的致病因子（），其在低温下表现出延长寿命的现象。为了探究这一现象的分子机制，我们尝试对来源于的环鸟苷酸（cGMP）信号通路中的三个基因的分子特征、转录丰度和功能进行研究。从 中鉴定出三个 cGMP 通路基因。利用生物信息学软件分析了这三个推测蛋白的特征。采用 RNA 干扰（RNAi）技术研究了这三个基因在耐冷性中的功能。结果表明，Bx-DAF-11 编码蛋白具有腺苷酸和鸟苷酸环化酶催化结构域，表明其具有结合细胞外配体和合成 cGMP 的能力。Bx-TAX-2 和 Bx-TAX-4 均具有环核苷酸结合域和离子转运蛋白域，表明它们是 cGMP 门控离子通道。在低温下， 、 和 的转录水平增加。三个基因沉默的 的存活率在低温下显著降低。本研究表明，cGMP 信号通路在 低温诱导的寿命延长中起关键作用。
