Wang Tingliang, Hill Ryan C, Dzieciatkowska Monika, Zhu Lian, Infante Aniello M, Hu Gangqing, Hansen Kirk C, Pei Ming
Stem Cell and Tissue Engineering Laboratory, Department of Orthopedics, West Virginia University, Morgantown, WV, United States.
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital Affiliated with Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Front Cell Dev Biol. 2020 Apr 15;8:237. doi: 10.3389/fcell.2020.00237. eCollection 2020.
Adult stem cells have unique properties in both proliferation and differentiation preference. In this study, we hypothesized that adipose stem cells have a depot-dependent lineage preference. Four rabbits were used to provide donor-matched adipose stem cells from either subcutaneous adipose tissue (ScAT) or infrapatellar fat pad (IPFP). Proliferation and multi-lineage differentiation were evaluated in adipose stem cells from donor-matched ScAT and IPFP. RNA sequencing (RNA-seq) and proteomics were conducted to uncover potential molecular discrepancy in adipose stem cells and their corresponding matrix microenvironments. We found that stem cells from ScAT exhibited significantly higher proliferation and adipogenic capacity compared to those from donor-matched IPFP while stem cells from IPFP displayed significantly higher chondrogenic potential compared to those from donor-matched ScAT. Our findings are strongly endorsed by supportive data from transcriptome and proteomics analyses, indicating a site-dependent lineage preference of adipose stem cells.
成体干细胞在增殖和分化偏好方面具有独特的特性。在本研究中，我们假设脂肪干细胞具有依赖于储存部位的谱系偏好。使用了4只兔子来提供来自皮下脂肪组织（ScAT）或髌下脂肪垫（IPFP）的供体匹配的脂肪干细胞。对来自供体匹配的ScAT和IPFP的脂肪干细胞的增殖和多谱系分化进行了评估。进行了RNA测序（RNA-seq）和蛋白质组学分析，以揭示脂肪干细胞及其相应基质微环境中的潜在分子差异。我们发现，与来自供体匹配的IPFP的干细胞相比，来自ScAT的干细胞表现出显著更高的增殖和成脂能力，而与来自供体匹配的ScAT的干细胞相比，来自IPFP的干细胞表现出显著更高的软骨生成潜力。我们的研究结果得到了转录组和蛋白质组学分析的支持数据的有力支持，表明脂肪干细胞具有位点依赖性的谱系偏好。
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