Chu Dinh-Toi, Nguyen Thi Phuong Thuy, Tien Nguyen Le Bao, Tran Dang Khoa, Minh Le Bui, Thanh Vo Van, Gia Anh Pham, Pham Van Huy, Thi Nga Vu
Faculty of Biology, Hanoi National University of Education, Hanoi 100000, Vietnam.
School of Odonto Stomatology, Hanoi Medical University, Hanoi 100000, Vietnam.
J Clin Med. 2019 Jun 26;8(7):917. doi: 10.3390/jcm8070917.
Adipose tissue stem cells (ASCs), known as multipotent stem cells, are most commonly used in the clinical applications in recent years. Adipose tissues (AT) have the advantage in the harvesting, isolation, and expansion of ASCs, especially an abundant amount of stem cells compared to bone marrow. ASCs can be found in stromal vascular fractions (SVF) which are easily obtained from the dissociation of adipose tissue. Both SVFs and culture-expanded ASCs exhibit the stem cell characteristics such as differentiation into multiple cell types, regeneration, and immune regulators. Therefore, SVFs and ASCs have been researched to evaluate the safety and benefits for human use. In fact, the number of clinical trials on ASCs is going to increase by years; however, most trials are in phase I and II, and lack phase III and IV. This systemic review highlights and updates the process of the harvesting, characteristics, isolation, culture, storage, and application of ASCs, as well as provides further directions on the therapeutic use of ASCs.
脂肪组织干细胞（ASCs），作为多能干细胞，近年来在临床应用中最为常用。脂肪组织（AT）在ASCs的采集、分离和扩增方面具有优势，尤其是与骨髓相比，其干细胞数量丰富。ASCs存在于基质血管成分（SVF）中，而SVF很容易从脂肪组织的解离中获得。SVF和培养扩增的ASCs都表现出干细胞特性，如分化为多种细胞类型、再生和免疫调节。因此，人们对SVF和ASCs进行了研究，以评估其对人类使用的安全性和益处。事实上，关于ASCs的临床试验数量逐年增加；然而，大多数试验处于I期和II期，缺乏III期和IV期试验。本系统综述突出并更新了ASCs的采集、特性、分离、培养、储存和应用过程，并为ASCs的治疗应用提供了进一步的方向。
