Liu Yiming, Zhao Yun, Han Mei, Zhang Yuqiu, Mi Fanglin, Wang Bing
Dept. of Stomatology, North Sichuan Medical College, Nanchong 637000, China.
Dept. of Stomatology, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2022 Oct 1;40(5):522-531. doi: 10.7518/hxkq.2022.05.004.
This study aimed to prepare functional composite electrospinning fibrous membranes with the functions of antifibrosis and bacteriostasis, as well as to explore its repair effect on rat jaw defect.
Poly-(lactic acid-co-glycolic acid) fibrous membrane loaded with cyclic-arginine-glycine-aspartic acid sequence, ornidazole, and nanohydroxyapatite (n-HA) was prepared by electrospinning as the functional layer (GBRL) for adhering onto defective bone. A barrier layer with the function of supporting and isolating different functional layers was prepared by tape-casting method. Poly(p-dioxanone-co-l-phenylalanine) fiber membrane with the function of inhibiting fibrosis was prepared by electrospinning technology as the antifibrosis layer (AFL). The morphology of the composite membrane was characterized by scanning electron microscopy. The effects of different functional layers on the proliferation of mouse osteoblast precursor cells (MC3T3-E1) and mouse fibroblasts (L929) were studied by cell-proliferation test (CCK-8 method). The inhibitory effect of composite membrane on the proliferation of was studied by bacteriostatic circle test. A rat mandibular-bone defect model was established to study the repair effect of composite fiber membrane on bone-defect tissue. This repair effect was compared with that of collagen oral-repair membrane commonly used in clinics.
The GBRL layer could support the proliferation of MC3T3-E1 cells, and the AFL layer could inhibit the proliferation of L929 cells. Composite membranes loaded with ornidazole could inhibit the proliferation of . Various composite membranes can induce the reconstruction of rat jaw defects, among which the composite membranes loaded with ornidazole and n-HA had the best repair effect, which was better than that of collagen oral-repair membrane.
The electrospun membrane loaded with ornidazole and n-HA as the composite fiber membrane of GBRL layer had excellent antibacterial and bone-tissue-regeneration activity. The effect was better than that of the commonly used collagen oral-repair membrane. Therefore, this material has great potential to induce bone regeneration for defects caused by periodontal diseases.
本研究旨在制备具有抗纤维化和抑菌功能的功能性复合静电纺丝纤维膜，并探讨其对大鼠颌骨缺损的修复效果。
通过静电纺丝制备负载环-精氨酸-甘氨酸-天冬氨酸序列、奥硝唑和纳米羟基磷灰石（n-HA）的聚（乳酸-乙醇酸共聚物）纤维膜作为功能层（GBRL），用于粘附到缺损骨上。采用流延法制备具有支撑和隔离不同功能层作用的阻挡层。通过静电纺丝技术制备具有抑制纤维化功能的聚（对二氧环己酮-左旋苯丙氨酸）纤维膜作为抗纤维化层（AFL）。用扫描电子显微镜对复合膜的形态进行表征。通过细胞增殖试验（CCK-8法）研究不同功能层对小鼠成骨细胞前体细胞（MC3T3-E1）和小鼠成纤维细胞（L929）增殖的影响。通过抑菌圈试验研究复合膜对[此处原文缺失具体细菌名称]增殖的抑制作用。建立大鼠下颌骨缺损模型，研究复合纤维膜对骨缺损组织的修复效果。将此修复效果与临床常用的胶原口腔修复膜进行比较。
GBRL层可支持MC3T3-E1细胞增殖，AFL层可抑制L929细胞增殖。负载奥硝唑的复合膜可抑制[此处原文缺失具体细菌名称]的增殖。各种复合膜均可诱导大鼠颌骨缺损的修复，其中负载奥硝唑和n-HA的复合膜修复效果最佳，优于胶原口腔修复膜。
负载奥硝唑和n-HA的静电纺丝膜作为GBRL层的复合纤维膜具有优异的抗菌和骨组织再生活性。效果优于常用的胶原口腔修复膜。因此，该材料在诱导牙周疾病所致缺损的骨再生方面具有巨大潜力。
