Department of Conservative Dentistry, Kyung Hee University Dental Hospital, Seoul, South Korea.
Environmnetal Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South Korea.
J Biomed Mater Res B Appl Biomater. 2020 Oct;108(7):2847-2856. doi: 10.1002/jbm.b.34616. Epub 2020 May 3.
The aim of this study was to investigate the effects of the incorporation of elastin-like polypeptide (ELP) on the adhesion maturation of mineral trioxide aggregates (MTA). Two types of ELPs (V125 and V125E8) were genetically synthesized. V125 consisted of 125 repeating pentapeptides (Val-Pro-Gly-Xaa-Gly) and V125E8 was functionalized with octaglutamic acid in the C-terminus of V125; both were diluted to 10 wt% in solution. Three 1.5 mm diameter holes in dentin discs were filled with MTA mixed with either a solution of ELP or deionized water. Push-out bond strength tests were performed following storage in simulated body fluid (SBF) for 1, 2, 4, and 8 weeks (n = 12). The interface between dentin and MTA was observed via scanning electron microscopy (SEM). The maturation of MTA was evaluated with a stereoscopic microscope and SEM. The incorporation of a specific ELP (V125E8) significantly increased the bond strength of MTA to dentin with regard to every maturation period (p < 0.05). The bond strength of MTA also significantly increased with a longer maturation time irrespective of ELP incorporation (p < 0.05). V125E8-incorporated MTA exhibited a more intimate interface with dentin compared to the other groups. More spindle-shaped crystal structures and thicker crystals were observed in all MTA mixtures as the storage duration increased even though V125E8 exhibited fewer crystal structures on the surface. Within the limitations of this study, the incorporation of V125E8 increased the adhesive properties of MTA and the maturation of MTA occurred regardless of ELP incorporation.
本研究旨在探讨弹性蛋白样多肽（ELP）的掺入对矿化三氧化物凝聚体（MTA）黏结成熟的影响。合成了两种类型的 ELP（V125 和 V125E8）。V125 由 125 个重复五肽（Val-Pro-Gly-Xaa-Gly）组成，V125E8 在 V125 的 C 末端带有 8 个谷氨酸；两者均以 10wt%的浓度稀释在溶液中。在牙本质圆盘上钻 3 个 1.5mm 直径的孔，用 MTA 混合物填充，混合物中加入 ELP 溶液或去离子水。在模拟体液（SBF）中储存 1、2、4 和 8 周后进行推出粘结强度测试（n = 12）。通过扫描电子显微镜（SEM）观察牙本质和 MTA 之间的界面。通过立体显微镜和 SEM 评估 MTA 的成熟度。特定 ELP（V125E8）的掺入显著提高了 MTA 与牙本质之间的粘结强度，在每个成熟阶段均有统计学意义（p < 0.05）。无论是否掺入 ELP，MTA 的粘结强度均随成熟时间的延长而显著增加（p < 0.05）。与其他组相比，掺入 V125E8 的 MTA 与牙本质之间的界面更紧密。随着储存时间的延长，所有 MTA 混合物中的纺锤形晶体结构增多，晶体变厚，尽管 V125E8 在表面上显示出较少的晶体结构。在本研究的限制内，V125E8 的掺入增加了 MTA 的粘结性能，且无论是否掺入 ELP，MTA 均发生成熟。
