玫瑰红苯肼/曙红 Y-金-聚吡咯杂化纳米材料：一种超高载量的双光敏纳米体系的设计。
(Rose Bengal)/(Eosin Yellow)-Gold-Polypyrrole Hybrids: A Design for Dual Photo-Active Nano-System with Ultra-High Loading Capacity.
机构信息
Department of Medical Applications of Laser, Pharmaceutical Nano-Technology Unit, National Institute of Laser Enhanced Sciences (NILES), Cairo University, Giza, Egypt.
Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
出版信息
Drug Des Devel Ther. 2021 Dec 14;15:5011-5023. doi: 10.2147/DDDT.S338922. eCollection 2021.
PURPOSE
Enhancement of the photodynamic/photothermal efficiency of two water-soluble dyes, rose bengal (RB) and eosin yellow (EY), via conjugation to a polymeric nano-system gold-polypyrrole nanoparticle (AuPpy NPs).
METHODOLOGY
A multi-step synthesis method and an in situ one-pot synthesis method were used. Loading percentage, particle size, zeta potential, morphology, UV-Vis-NIR spectrophotometry and in vitro photothermal activity were measured. Then, both hybrid nanocomposites were examined for their cytotoxicity and photocytotoxicity on HepG2 cell line as a model for cancer cells.
RESULTS
Dyes loaded in the traditional multi-step method did not exceed 9% w/w, while in the one-pot synthesis method they reached ~67% w/w and ~75% w/w for EY-AuPpy NPs and RB-AuPpy NPs, respectively. UV-Vis-NIR spectrophotometry showed that both nano-systems exhibited intense absorption in the NIR region. The mean size of the nanoparticles was ~31.5 nm (RB-AuPpy NPs) and ~33.6 nm (EY-AuPpy NPs) with zeta potential values of -26.5 mV and -33 mV, respectively. TEM imaging revealed the morphology of both hybrids, showing ultra-nano spherical-shaped gold cores in the case of RB-AuPpy NPs, and different shapes of larger gold cores in the case of EY-AuPpy NPs, both embedded in the polymer film. Conjugation to AuPpy was found to significantly reduce the dark cytotoxicity of both RB and EY, preserving the photocytotoxicity of EY and enhancing the photocytotoxicity of RB.
CONCLUSION
Gold-polypyrrole nanoparticles represent an effective delivery system to improve the photodynamic and photothermal properties of RB and EY. The in situ one-pot synthesis method provided a means to greatly increase the loading capacity of AuPpy NPs. While both hybrid nanocomposites exhibited greatly diminished dark cytotoxicity, RB-AuPpy NPs showed significantly enhanced photocytotoxicity compared to the free dyes. This pattern enables the safe use of both dyes in high concentrations with sustained action, reducing dose frequency and side effects.
目的
通过与聚合物纳米系统金-聚吡咯纳米粒子（AuPpy NPs）结合，增强两种水溶性染料玫瑰红（RB）和曙红黄（EY）的光动力/光热效率。
方法
采用多步合成法和原位一锅合成法。测量了负载百分比、粒径、Zeta 电位、形态、紫外-可见-近红外分光光度法和体外光热活性。然后，以 HepG2 细胞系为癌细胞模型，研究了两种杂化纳米复合材料对其细胞毒性和光细胞毒性的影响。
结果
用传统的多步法负载的染料不超过 9%w/w，而在一锅合成法中，EY-AuPpy NPs 和 RB-AuPpy NPs 的负载量分别达到67%w/w 和75%w/w。紫外-可见-近红外分光光度法表明，两种纳米系统在近红外区域都表现出强烈的吸收。纳米粒子的平均粒径约为31.5nm（RB-AuPpy NPs）和33.6nm（EY-AuPpy NPs），Zeta 电位值分别为-26.5mV 和-33mV。TEM 成像显示了两种杂化物的形态，在 RB-AuPpy NPs 的情况下显示出超纳米球形金核，而在 EY-AuPpy NPs 的情况下显示出不同形状的较大金核，两者都嵌入在聚合物膜中。与 AuPpy 的结合被发现显著降低了 RB 和 EY 的暗毒性，同时保留了 EY 的光细胞毒性，并增强了 RB 的光细胞毒性。
结论
金-聚吡咯纳米粒子代表了一种有效的递药系统，可提高 RB 和 EY 的光动力和光热特性。原位一锅合成法提供了一种极大增加 AuPpy NPs 负载能力的方法。虽然两种杂化纳米复合材料的暗毒性都大大降低，但与游离染料相比，RB-AuPpy NPs 的光细胞毒性显著增强。这种模式使两种染料能够以高浓度安全使用，并具有持续作用，从而减少剂量频率和副作用。
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